Upregulation of GAPDH and Tom20 in C6 gliomas. a A section of rat brain stained with hematoxilin and eosin to show a glioma produced by implantation of C6 cells in the right caudate nucleus. In this case, cells also grew in the region of the syringe needle track through the cortex. (b–d). A small glioma labeled by bisbenzamide (b) and with antibodies against Tom20 (c) and GAPDH (d). The brightness and contrast have been increased. e Illustration of a strip perpendicular to the rim of a glioma (labeled with bisbenzamide) and along which intensity profiles were measured. f, g Tom20 and GAPDH fluorescence along such a strip (barely visible without enhancement). The graphs show the raw intensity values given by ImageJ.