Table 3 Transformation efficiency
From: Transient and stable transformation of Ceratopteris richardii gametophytes
# | Enzyme treatment [1.5% (w/v) cellulase] | Agrobacterium co-incubation (GFP or GUS) | Total gametophytes | Survivors (0.5× MS + 100 mg L−1 cefotaxime + 2.5 mg L−1 hygromycin) | Efficiency |
|---|---|---|---|---|---|
1 | Yes | No | ~21,000a | 8 | ~0.038% |
2 | No | No | ~21,000a | 2 | ~0.009% |
3 | No | GFP | ~21,000a | 16 | ~0.076% |
5 | Yes | GFP | 38,364 | 176 | 0.459% |