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Table 3 Transformation efficiency

From: Transient and stable transformation of Ceratopteris richardii gametophytes

# Enzyme treatment [1.5% (w/v) cellulase] Agrobacterium co-incubation (GFP or GUS) Total gametophytes Survivors (0.5× MS + 100 mg L−1 cefotaxime + 2.5 mg L−1 hygromycin) Efficiency
1 Yes No ~21,000a 8 ~0.038%
2 No No ~21,000a 2 ~0.009%
3 No GFP ~21,000a 16 ~0.076%
5 Yes GFP 38,364 176 0.459%
  1. aApproximate number of gametophytes of three equal portions from one culture.