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Table 1 qRT-PCR primer sets and reaction efficiencies

From: Na+/K+-ATPase α1 mRNA expression in the gill and rectal gland of the Atlantic stingray, Dasyatis sabina, following acclimation to increased salinity

Gene Primer/probe Sequence Efficiency (%)
18S Forward primer 5′-GTTAATTCCGATAACGAACGAGACTC-3′ 100
Reverse primer 5′-ACAGACCTGTTATTGCTCAATCTCGTG-3′
atp1a1 a Forward primer 5′-CTCTCACTCATCTTGGGATACAG-3′ 89
Reverse primer 5′-GGCATCTCCAGCAACACTT-3′
  1. aChoe et al. [15].
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