Table 1 qRT-PCR primer sets and reaction efficiencies
Gene | Primer/probe | Sequence | Efficiency (%) |
|---|---|---|---|
18S | Forward primer | 5′-GTTAATTCCGATAACGAACGAGACTC-3′ | 100 |
Reverse primer | 5′-ACAGACCTGTTATTGCTCAATCTCGTG-3′ | ||
atp1a1 a | Forward primer | 5′-CTCTCACTCATCTTGGGATACAG-3′ | 89 |
Reverse primer | 5′-GGCATCTCCAGCAACACTT-3′ |