Na + /K + -ATPase α1 mRNA expression in the gill and rectal gland of the Atlantic stingray, Dasyatis sabina , following acclimation to increased salinity

Table 1 qRT-PCR primer sets and reaction efficiencies

Gene	Primer/probe	Sequence	Efficiency (%)
18S	Forward primer	5′-GTTAATTCCGATAACGAACGAGACTC-3′	100
18S	Reverse primer	5′-ACAGACCTGTTATTGCTCAATCTCGTG-3′	100
atp1a1 ^a	Forward primer	5′-CTCTCACTCATCTTGGGATACAG-3′	89
atp1a1 ^a	Reverse primer	5′-GGCATCTCCAGCAACACTT-3′	89

ISSN: 1756-0500