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Fig. 3 | BMC Research Notes

Fig. 3

From: Insulin and IGF-1, but not 17β-estradiol, alter the subcellular localization of MIER1α in MCF7 breast carcinoma cells

Fig. 3

IGF-1 reduces nuclear localization of MIER1α. MCF7 cells were transfected with myc-tagged mier1α and treated 24 h later with either vehicle (panels a–d) or 10 ng/ml IGF-1 (panels e–l). Cells were fixed 4 h after the addition of IGF-1 and localization was analyzed by confocal microscopy, as in Fig. 1. A Compiled Z-stacks showing nuclei (DAPI; panels a, e, i), MIER1α (AlexaFluor-488, panels b, f, j), HDAC1 (AlexaFluor-647, panels c, g), HDAC2 (AlexaFluor-647, panel k) and merged 488 and 647 channels (panels d, h, l). B Histogram showing the results of two independent experiments; the MIER1α localization pattern of >1000 cells was scored. Plotted is the percentage of cells in each category ±SD; Asterisk indicates that the difference was statistically significant, p < 0.05 (Fisher’s exact test). Note that nuclear localization of HDAC1 and HDAC2 was unaffected by IGF-1

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BMC Research Notes

ISSN: 1756-0500

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