Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt

Olsvik, Pål A.; Urke, Henning A.; Nilsen, Tom O.; Ulvund, John B.; Kristensen, Torstein

doi:10.1186/s13104-015-1342-2

Table 3 PCR primers, accession or contig numbers, amplicon sizes and PCR efficiencies

From: Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt

Gene product	Gene name	Marker for	Accession no.	Forward primer	Reverse primer	Amplicon size (bp)	PCR efficiency ^liver/gills
Catalase	CAT	Oxidative stress	BT059457	CCCAAGTCTTCATCCAGAAACG	CGTGGGCTCAGTGTTGTTGA	123	2.03
Glutathione peroxidase 1	GPX1	Oxidative stress	DW566563	GCCCACCCCTTGTTTGTGTA	AGACAGGGCTCCACATGATGA	103	1.96
Manganese superoxide dismutase	MNSOD	Oxidative stress	DY718412	GTTTCTCTCCAGCCTGCTCTAAG	CCGCTCTCCTTGTCGAAGC	227	1.87
Heme oxygenase 1	HMOX1	Iron metabolism/oxidative stress	BG936101	AGCAGATTAAAGCTGTAACCAAGGA	GCCAGCATCAGCTCAGTGTTC	64	2.07/1.75
Heat shock protein 70	HSP70	Protein folding/oxidative stress	BG933934	CCCCTGTCCCTGGGTATTG	CACCAGGCTGGTTGTCTGAGT	121	2.04/2.06
Metallothionein B	MTB	Stress/oxidative stress	CK990996	TGAATAAAGAAGCGCGATCAAA	CTGGTGCATGCGCAGTTG	111	1.87
Hypoxia-inducible factor 1A	HIF1A	Oxidative stress	DY708816	CCACCTCATGAAGACCCATCA	TCTCCACCCACACAAAGCCT	101	1.94
Cytochrome P450 1A	CYP1A	Detoxification	AF364076	TGGAGATCTTCCGGCACTCT	CAGGTGTCCTTGGGAATGGA	101	2.06/1.92
Cytochrome P450 3A	CYP3A	Detoxification	DQ361036	ACTAGAGAGGGTCGCCAAGA	TACTGAACCGCTCTGGTTTG	146	2.00
Glutathione S-transferase P1	GSTP1	Detoxification/oxidative stress	BQ036247	ATTTTGGGACGGGCTGACA	CCTGGTGCTCTGCTCCAGTT	81	2.11
Tumor suppressor p53	P53	DNA damage	BT058777	CTCGCCAGACCTGAACAAGTT	ATAGATGGCCAGGGCTCGTA	112	2.34
Apoptosis regulator Bcl-X	BCLX	DNA damage/apoptosis	NM_001141086	GCCTGGACGCAGTGAAAGAG	GGACGGCGTGATGTGTAGCT	107	1.99
Insulin-like growth factor binding protein 1B	IGFBP1B	Anti-growth	AY662657	GAGGACCAGGGACAAGAGAAAGT	GCACCCTCATTTTTGGTGTCA	101	1.98
Mitogen-activated kinase 1	MAPK1	MAP kinase activity	EF101948	TCAATCTGGAGAAGGAGCTCGTA	CTACCTGCCGTAGCTCTTCGAT	51	1.81
Caspase 3B	CASP3B	Apoptosis	DQ008069	AGCCGATTCGGTGTTAAAAGG	CCGGAGGCTTAGCGTCTACTT	107	2.08
Tumor necrosis factor receptor superfamily 1A	TNFRSF1A	Infammation/apoptosis	NM_001141773	AAGACCTGCCTCCGTTGTACA	CTGAGGCACTCCCGTGTTTC	140	1.96
B-actin	ACTB	Reference gene	BG933897	CCAAAGCCAACAGGGAGAA	AGGGACAACACTGCCTGGAT	92	1.94/1.85
Eukaryotic translation elongation factor 1 alpha B	EEF1AB	Reference gene	BG933853	TGCCCCTCCAGGATGTCTAC	CACGGCCCACAGGTACTG	59	2.00
Ribosomal protein L13	RPL13	Reference gene	NM_001141291	CCAATGTACAGCGCCTGAAA	CGTGGCCATCTTGAGTTCCT	110	2.02/2.05
60S ribosomal protein L40	UBA52	Reference gene	GO054675	GATCTTCGCTGGCAAACAACT	CGAAGACGCAGCACAAGATG	93	/2.12

Back to article page

ISSN: 1756-0500

Contact us

Submission enquiries: bmcresearchnotes@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Research Notes

Contact us