Skip to main content

Table 2 Kinetic parameters for the interaction between TetR and promoters L09, L10, L11, and L12

From: Analysis of the leakage of gene repression by an artificial TetR-regulated promoter in cyanobacteria

Promoter

k 1 (s−1 M−1)a (pk1)

k −1 (s−1)a (pk−1)

K D (M)b (pK D )

L09

2 × 105 (−5.2 ± 0.1)

1 × 10−3 (3.0 ± 0.1)

6 × 10−9 (8.2 ± 0.1)

L10

2 × 105 (−5.3 ± 0.1)

1 × 10−3 (3.0 ± 0.1)

5 × 10−9 (8.3 ± 0.1)

L11

2 × 105 (−5.3 ± 0.3)

1 × 10−3 (3.0 ± 0.1)

6 × 10−9 (8.2 ± 0.2)

L12

2 × 105 (−5.3 ± 0.2)

2 × 10−3 (2.8 ± 0.1)

8 × 10−9 (8.1 ± 0.1)

  1. aAssociation (k 1) and dissociation rate constants (k −1) are shown together with the pk1 and pk−1 values and the respective standard deviations
  2. bThe equilibrium dissociation constants (K D ) are shown together with the respective pK D values and standard deviations. The data is based on three replicate experiments (±SD)
Back to article page

BMC Research Notes

ISSN: 1756-0500

Contact us