Fig. 2

Amino acid alignment of 25 predicted NS sequences compared to A/equine/Miami/1963. A/equine/Miami/1/1963, ABY81497; A/equine/Nador/1/1997, AFN69204; A/equine/Essaouira/2/2004, AFJ69907; A/equine/Essaouira/3/2004, AFJ69911; A/eq/LaPlata/93, AAC31270; A/equine/Aboyne/1/2005, ACH95651; A/equine/Ahmedabad/1/2009, ADB45168; A/equine/Almaty/26/2007, ADE21678; A/equine/Austria/421/1992, ACD85324; A/equine/California/4537/1997, ACA96805; A/equine/Cheshire/1/2007, ACH95645; A/equine/Grobois/1/1998, AAP92165; A/equine/Italy/1199/1992, ACD85313; A/equine/Kentucky/2/1980, ACF22120; A/equine/Kentucky/7/2007, ACH95629; A/equine/Newmarket/1/2007, ACH95607; A/equine/North Carolina/152429/2002, ACZ47169; A/equine/Ohio/1/2003, ABA42431; A/equine/Pulawy/1/2005, AIW60784; A/equine/Xuzhou/01/2013, AHA98359; A/eq/Kentucky/92, AAC31266; A/equine/Cheshire/1/2006, ACH95605; A/equine/Kentucky/1/1997, ACH95653; A/equine/Lincolnshire/1/2006, ACH95599; A/equine/Yvelines/2136/1989, AAC31256. Topology of α-helix and β-strands of NS1 protein: α1(4–24), α2 (31–50), α3 (54–70), α4(95–99), α5(171–188), α6 (195–205), β1(88–91), β2 (107–112), β3 (115–120), β4(126–136), β5(142–151), β6 (157–162), β7 (191–194) [15]