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Table 1 Characterisation and PCR conditions for 10 microsatellite loci in the Cape horseshoe bat, Rhinolophus capensis

From: Development and characterization of 10 microsatellite markers in the Cape horseshoe bat, Rhinolophus capensis (Chiroptera, Rhinolophidae) and cross-amplification in southern African Rhinolophus species

Locus Repeat motif Primer sequences (5′–3′) Dye Temp. annealing (°C) Panel Expected product size (bp) Observed size range (bp) Population n NA NE HO HE PHW PIC GenBank accession number
RCA1 (ATAG)10 F: TGG GAA ATC TCC AAA CCT TCC VIC 55 1 560 554–582 Table farm 19 8 4.57 0.737 0.781 0.092 0.789 KP842268
   R: GGG CTG CCT CAA CAT AAT ACC       Heidehof 20 6 4.87 0.700 0.795 0.288   
RCA2 (AG)32 F: GTG AAC CTA CTT GCT TGA CTC ACC 6-FAM 54 1 519 492–538 Table farm 14 12 6.87 0.786 0.855 0.030 0.903 KP842262
   R: TGG CCA GTT GAA GAC AGT CTC C       Heidehof 17 14 9.63 0.529 0.896 0.0001*   
RCA3 (TAGA)14 F: AGC AGC CTC CAT AAT AAC ATA AGC VIC 50 2 420 398–418 Table farm 20 5 3.37 0.700 0.704 0.756 0.708 KP842266
   R: AAC TCT TAT CCT AAT CTC ACC TCC       Heidehof 20 6 4.08 0.750 0.755 0.657   
RCA4 (TG)20 F: GTA TGA GTC CTT TGT CAG ATA GAG G 6-FAM 52 2 375 374–384 Table farm 19 6 3.48 0.632 0.713 0.008 0.685 KP842263
   R: TGG TAA CAA TGC AGT GTA ATG G       Heidehof 20 5 3.68 0.850 0.729 0.667   
RCA5 (TC)26 F: GCT CAT GCA TGG TAG GCA ATG G VIC 57 1 311 288–312 Table farm 18 4 2.48 0.833 0.597 0.121 0.631 KP842265
   R: AGA CAT TCA GGT CTC CAC ACT CC       Heidehof 18 7 3.17 0.667 0.685 0.506   
RCA6 (GATA)12 F: GCA GAG CAG AAT GTC AAC ATC C PET 52 2 258 238–262 Table farm 19 4 3.04 0.632 0.672 0.122 0.634 KP842264
   R: AGT AGC CAT CCC TTT CAA TCC       Heidehof 20 5 3.21 0.850 0.689 0.572   
RCA7 (ATAG)10 F: GCC TTC ATA GAG CTG ATA TGT TGG NED 56 1 234 228–244 Table farm 20 5 3.90 0.900 0.744 0.359 0.690 KP842267
   R: GAG CCT GCC ATT CTA CAA TCC       Heidehof 20 4 3.49 0.800 0.714 0.893   
RDE1 (TCTA)11 F: TGT TAC ATC TTG GTC CCT TGA GG NED 53 1 400 394–410 Table farm 20 5 3.82 0.650 0.739 0.710 0.685 KP842271
   R: GCT GCA TAT GGG CTG AGA TAA AC       Heidehof 20 4 3.37 0.850 0.704 0.326   
RDE2 (AC)25 F: TCA CTC ACT TCT CTC GAC TCC PET 53 1 400 387–409 Table farm 18 7 3.10 0.500 0.677 0.106 0.642 KP842269
   R: AGA CCA CAA CTC TTA ACT CTG C       Heidehof 20 7 2.87 0.450 0.653 0.006   
RDE3 (TC)24 F: CCT TCT CAG TTA CTA TTA CCT CAC C NED 53 2 336 332–388 Table farm 19 12 9.13 0.737 0.891 0.059 0.872 KP842270
   R: TGC TCT AAC TCC TCA CAC ACC       Heidehof 20 11 5.51 0.750 0.840 0.514   
  1. Number of individuals genotyped (n), number of alleles observed per locus (NA), effective number of alleles (NE), observed (HO), and expected heterozygosity (HE), Probability of deviation from Hardy–Weinberg Equilibrium (PHW), Polymorphic information content (PIC)
  2. * P values significant after Bonferroni correction at α = 0.05 (p < 0.0025)