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Table 1 Characterisation and PCR conditions for 10 microsatellite loci in the Cape horseshoe bat, Rhinolophus capensis

From: Development and characterization of 10 microsatellite markers in the Cape horseshoe bat, Rhinolophus capensis (Chiroptera, Rhinolophidae) and cross-amplification in southern African Rhinolophus species

Locus

Repeat motif

Primer sequences (5′–3′)

Dye

Temp. annealing (°C)

Panel

Expected product size (bp)

Observed size range (bp)

Population

n

NA

NE

HO

HE

PHW

PIC

GenBank accession number

RCA1

(ATAG)10

F: TGG GAA ATC TCC AAA CCT TCC

VIC

55

1

560

554–582

Table farm

19

8

4.57

0.737

0.781

0.092

0.789

KP842268

  

R: GGG CTG CCT CAA CAT AAT ACC

     

Heidehof

20

6

4.87

0.700

0.795

0.288

  

RCA2

(AG)32

F: GTG AAC CTA CTT GCT TGA CTC ACC

6-FAM

54

1

519

492–538

Table farm

14

12

6.87

0.786

0.855

0.030

0.903

KP842262

  

R: TGG CCA GTT GAA GAC AGT CTC C

     

Heidehof

17

14

9.63

0.529

0.896

0.0001*

  

RCA3

(TAGA)14

F: AGC AGC CTC CAT AAT AAC ATA AGC

VIC

50

2

420

398–418

Table farm

20

5

3.37

0.700

0.704

0.756

0.708

KP842266

  

R: AAC TCT TAT CCT AAT CTC ACC TCC

     

Heidehof

20

6

4.08

0.750

0.755

0.657

  

RCA4

(TG)20

F: GTA TGA GTC CTT TGT CAG ATA GAG G

6-FAM

52

2

375

374–384

Table farm

19

6

3.48

0.632

0.713

0.008

0.685

KP842263

  

R: TGG TAA CAA TGC AGT GTA ATG G

     

Heidehof

20

5

3.68

0.850

0.729

0.667

  

RCA5

(TC)26

F: GCT CAT GCA TGG TAG GCA ATG G

VIC

57

1

311

288–312

Table farm

18

4

2.48

0.833

0.597

0.121

0.631

KP842265

  

R: AGA CAT TCA GGT CTC CAC ACT CC

     

Heidehof

18

7

3.17

0.667

0.685

0.506

  

RCA6

(GATA)12

F: GCA GAG CAG AAT GTC AAC ATC C

PET

52

2

258

238–262

Table farm

19

4

3.04

0.632

0.672

0.122

0.634

KP842264

  

R: AGT AGC CAT CCC TTT CAA TCC

     

Heidehof

20

5

3.21

0.850

0.689

0.572

  

RCA7

(ATAG)10

F: GCC TTC ATA GAG CTG ATA TGT TGG

NED

56

1

234

228–244

Table farm

20

5

3.90

0.900

0.744

0.359

0.690

KP842267

  

R: GAG CCT GCC ATT CTA CAA TCC

     

Heidehof

20

4

3.49

0.800

0.714

0.893

  

RDE1

(TCTA)11

F: TGT TAC ATC TTG GTC CCT TGA GG

NED

53

1

400

394–410

Table farm

20

5

3.82

0.650

0.739

0.710

0.685

KP842271

  

R: GCT GCA TAT GGG CTG AGA TAA AC

     

Heidehof

20

4

3.37

0.850

0.704

0.326

  

RDE2

(AC)25

F: TCA CTC ACT TCT CTC GAC TCC

PET

53

1

400

387–409

Table farm

18

7

3.10

0.500

0.677

0.106

0.642

KP842269

  

R: AGA CCA CAA CTC TTA ACT CTG C

     

Heidehof

20

7

2.87

0.450

0.653

0.006

  

RDE3

(TC)24

F: CCT TCT CAG TTA CTA TTA CCT CAC C

NED

53

2

336

332–388

Table farm

19

12

9.13

0.737

0.891

0.059

0.872

KP842270

  

R: TGC TCT AAC TCC TCA CAC ACC

     

Heidehof

20

11

5.51

0.750

0.840

0.514

  
  1. Number of individuals genotyped (n), number of alleles observed per locus (NA), effective number of alleles (NE), observed (HO), and expected heterozygosity (HE), Probability of deviation from Hardy–Weinberg Equilibrium (PHW), Polymorphic information content (PIC)
  2. * P values significant after Bonferroni correction at α = 0.05 (p < 0.0025)
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BMC Research Notes

ISSN: 1756-0500

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