Fig. 1

Pulmonary inflammation and injury after aspiration. IT injections of saline, acid, or acid + particles were given to groups of mice (No CLP). In additional groups of mice, cecal ligation and puncture was performed followed by identical IT injections. CLP was performed either immediately before the IT injection (0 Hours) or preceded the IT injections by 12 or 48 h (12 Hours or 48 Hours). All mice were euthanized at 6 h post-IT injection and bronchoalveolar lavage was performed. a Neutrophil counts from BAL fluid. b Albumin levels in BAL fluid. n = 10–12/group. Results are expressed as mean ± SEM. *p < 0.05, S = p < 0.05 compared to No CLP Saline group, A = p < 0.05 compared to No CLP Acid group, P = p < 0.05 compared to No CLP Particles group; S0, A0, or P0 = p < 0.05 as compared to Saline, Acid or Particles group with 0 Hours interval between insults