Table 3 1e6 spike-in scheme and primer pairs per organism
From: Targeted amplification for enhanced detection of biothreat agents by next-generation sequencing
Organism | Genome equivalents/sample | No of primer pairs |
|---|---|---|
Human | 3000 | N/A |
Yersinia pestis | 1,000,000 | 99 |
Francisella tularensis | 1,000,000 | 24 |
Bacillus anthracis | 1,000,000 | 66 |
Burkholderia mallei | 0 | 5 |
Burkholderia pseudomallei | 1,000,000 | 124 |
Salmonella enterica | 0 | 46 |
Thermotoga maritima | 10,000 | 50 |