1
|
V1F–V1R
|
rsbV1
|
Confirms intron insertion (primers flank insertion site)
|
2
|
incAF–incAR
|
incA
|
Confirms intron insertion (primers flank insertion site)
|
3
|
hyp08F–hyp08R
|
cryptic plasmid
|
Confirms that transformation has not resulted in loss of the native, cryptic plasmid
|
4
|
incAF–GIIR
|
Connects the 5′ region of incA to the intron
|
Confirms intron insertion and orientation
|
5
|
incAR–GIIF
|
Connects the 3′ region of incA to the intron
|
Confirms intron insertion and orientation
|
6
|
GIIF–GIIR
|
Intron
|
Confirms intron presence
|
7
|
incAF–aadAR
|
Connects incA to the aadA marker
|
Confirms marker type
|
8
|
catF–catR
|
Intron donor plasmid
|
Confirms loss of intron donor plasmid
|
9
|
V1F–GIIF
|
Connects the 5′ region of rsbV1 to the intron
|
Confirms intron insertion and orientation
|
10
|
V1R2–GIIR
|
Connects the 3′ region of rsbV1 to the intron
|
Confirms intron insertion and orientation
|
11
|
blaR2–VIR
|
Connects rsbV1 to the bla marker
|
Confirms marker type
|
12
|
blaF1–blaR1
|
Marker
|
Confirms presence of bla marker
|
13
|
aadAF–aadAR
|
Marker
|
Confirms presence of aadA marker
|