Fig. 1

Phylogenetic tree of SSIII SBD regions. The tree is based on the alignment of SBD regions from SSIII amino acid sequences obtained with PSI-Blast (shown in Additional Table 1). It was built by Maximum Parsimony method in MEGA 6.06 version [57] and it is rooted to A. niger amyloglucosidase (AspniGA) CBM20 (used as outgroup sequence). Phylogenies were determined by Bootstrap Analysis of 500 replicates in MEGA 6.06 version [59]. Branch lengths are proportional to distances. Bootstrap values are shown above branches Abbreviations of the source proteins: Ostlu, O. lucimarinus; Micsp, Micromonas sp. RCC299; Ostta, O. tauri; Micpu, Micromonas pusilla CCMP1545, Chlre, Chlamydomonas reinhardtii; Volca, Volvox carteri; Chlva, Chlorella variabilis, Zeama, Zea mays; Sorbi, Sorghum bicolor; Orysa, Oryza sativa; Musac, Musa acuminata; Soltu, Solanum tuberosum; Arath, Arabidopsis thaliana; Cucme, Cucumis melo; Glyma, Glycine max. Asterisk indicate hypothetical proteins names assigned by O. tauri SSIIIs homology. The schematic representation of SBD number and position (in grey color) from each SSIII protein is included on the right side of the figure