Fig. 1
From: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology
A schematic representation of the RAPID-M technology. The modified T7Term primer was mixed with the immobilization beads in a PCR microwell plate (a) to create beads-immobilized T7Term primer (b). The target DNA is directly immobilized to the beads by PCR amplification using beads-immobilized T7Term and soluble T7Prom to create high density immobilized DNA (HDID) in each well (c). The beads-immobilized DNA is translated to protein with the addition of cell-free reagents (d, e) and the protein is arrayed onto Ni–NTA slides using a microarray printer to produce high density protein microarray slides (HDS) (f)