Fig. 1

DNA integrity assayed by β-actin ddPCR. DNA templates were extracted from snap frozen SKBR-3 cells and cells treated with 10 % NBF or SCP. Three different fragment lengths from the same target gene β-actin were amplified. The sizes of three nested amplicons are 69 bp (a), 152 bp (b) and 420 bp (c), respectively. Mean copy numbers are shown with error bars indicating standard deviation, n = 10. *P < 0.005