Skip to main content

Table 4 Repartition of CNAs observed in CD19 and CD19+ cells. CD19+ cells presented twice as many CNAs as CD19 [83 CNAs (12–218) versus 42 (3–184)]

From: Persistent polyclonal binucleated B-cell lymphocytosis and MECOM gene amplification

CD19− CNAs—Total (gains/losses)
Chromosome UPN73 UPN71 UPN57 UPN136 UPN138 UPN99 UPN147 UPN105 Mean
1 2 (2/0) 0 (0/0) 4 (1/3) 1 (0/1) 3 (3/0) 1 (0/1) 0 (0/0) 17 (0/17) 3.5 (0.7/2.8)
2 3 (1/2) 1 (1/0) 0 (0/0) 2 (0/2) 2 (2/0) 0 (0/0) 0 (0/0) 24 (0/24) 4.0 (0.5/3.5)
3 2 (1/1) 0 (0/0) 3 (3/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 8 (1/7) 1.6 (0.6/1.0)
3p 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0.0 (0.0/0.0)
3q 2 (1/1) 0 (0/0) 3 (3/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 8 (1/7) 1.6 (0.6/1.0)
4 0 (0/0) 0 (0/0) 0 (0/0) 2 (1/1) 0 (0/0) 0 (0/0) 1 (1/0) 30 (1/29) 4.1 (0.4/3.7)
5 0 (0/0) 1 (0/1) 1 (0/1) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 15 (0/15) 2.3 (0.1/2.2)
6 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 11 (0/11) 1.5 (0.1/1.4)
7 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 1 (1/0) 12 (0/12) 1.8 (0.3/1.5)
8 1 (0/1) 0 (0/0) 0 (0/0) 1 (1/0) 3 (3/0) 0 (0/0) 0 (0/0) 6 (0/6) 1.4 (0.5/0.9)
9 0 (0/0) 4 (0/4) 3 (0/3) 0 (0/0) 4 (2/2) 1 (1/0) 0 (0/0) 7 (0/7) 2.4 (0.4/2.0)
10 1 (1/0) 1 (0/1) 1 (0/1) 0 (0/0) 1 (1/0) 0 (0/0) 1 (1/0) 7 (0/7) 1.5 (0.4/1.1)
11 0 (0/0) 2 (1/1) 0 (0/0) 0 (0/0) 2 (2/0) 0 (0/0) 1 (0/1) 10 (0/10) 1.9 (0.4/1.5)
12 2 (2/0) 0 (0/0) 1 (0/1) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 8 (1/7) 1.5 (0.5/1.0)
13 0 (0/0) 0 (0/0) 2 (0/2) 0 (0/0) 1 (0/1) 0 (0/0) 0 (0/0) 8 (1/7) 1.4 (0.1/1.3)
14 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 6 (1/5) 0.8 (0.1/0.7)
15 2 (2/0) 1 (1/0) 1 (0/1) 2 (0/2) 2 (1/1) 0 (0/0) 1 (1/0) 4 (1/3) 1.6 (0.9/0.7)
16 1 (1/0) 0 (0/0) 0 (0/0) 1 (1/0) 3 (1/2) 0 (0/0) 0 (0/0) 1 (0/1) 0.8 (0.4/0.4)
17 0 (0/0) 0 (0/0) 1 (0/1) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 4 (2/2) 0.8 (0.4/0.4)
18 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 1 (0/1) 0.2 (0.2/0.1)
19 0 (0/0) 1 (0/1) 0 (0/0) 0 (0/0) 0 (0/0) 1 (0/1) 1 (0/1) 1 (0/1) 0.5 (0.0/0.5)
20 1 (1/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 0 (0/0) 0 (0/0) 0.3 (0.3/0.0)
21 1 (0/1) 1 (0/1) 1 (0/1) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (0/1) 0.5 (0.0/0.5)
22 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 1 (1/0) 0.3 (0.3/0.0)
X 1 (1/0) 7 (6/1) 13 (13/0) 1 (0/1) 25 (24/1) 0 (0/0) 2 (1/1) 1 (0/1) 6.2 (5.6/0.6)
Y 1 (1/0) 1 (1/0) 2 (0/2) 0 (0/0) 2 (2/0) 0 (0/0) 0 (0/0) 1 (0/1) 0.9 (0.5/0.4)
Total 18 (13/5) 20 (10/10) 33 (18/15) 10 (3/7) 55 (48/7) 3 (1/2) 9 (6/3) 184 (9/175) 41.5 (13.5/28)
CD19+ CNAs—total (gains/losses)
Chromosome UPN73 UPN8 UPN57 UPN136 UPN127 UPN138 UPN99 UPN147 UPN105 Mean
1 20 (19/1) 0 (0/0) 0 (0/0) 4 (0/4) 0 (0/0) 0 (0/0) 5 (4/1) 8 (1/7) 0 (0/0) 5.0 (3.6/1.4)
2 22 (22/0) 2 (1/1) 0 (0/0) 2 (0/2) 0 (0/0) 1 (0/1) 3 (3/0) 12 (1/11) 0 (0/0) 5.7 (3.9/1.8)
3 20 (19/1) 4 (4/0) 26 (25/1) 0 (0/0) 46 (46/0) 1 (1/0) 2 (2/0) 5 (0/5) 113 (113/0) 25.0 (24.2/0.8)
3p 11 (11/0) 0 (0/0) 1 (0/1) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1.8 (1.7/0.1)
3q 9 (8/1) 4 (4/0) 25 (25/0) 0 (0/0) 46 (46/0) 1 (1/0) 2 (2/0) 5 (0/5) 113 (113/0) 23.2 (22.6/0.7)
4 9 (9/0) 0 (0/0) 2 (0/2) 5 (1/4) 0 (0/0) 1 (1/0) 0 (0/0) 10 (1/9) 0 (0/0) 4.0 (2.3/1.7)
5 14 (13/1) 0 (0/0) 0 (0/0) 2 (0/2) 0 (0/0) 0 (0/0) 0 (0/0) 5 (0/5) 1 (0/1) 3.4 (2.4/1.0)
6 10 (10/0) 1 (1/0) 0 (0/0) 1 (0/1) 0 (0/0) 1 (1/0) 1 (1/0) 5 (1/4) 0 (0/0) 2.9 (2.3/0.6)
7 12 (12/0) 1 (0/1) 0 (0/0) 1 (0/1) 1 (0/1) 0 (0/0) 1 (1/0) 6 (1/5) 0 (0/0) 2.4 (1.6/0.8)
8 13 (11/2) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (0/1) 1 (1/0) 2 (0/2) 0 (0/0) 2.3 (1.8/0.5)
9 14 (14/0) 0 (0/0) 0 (0/0) 4 (0/4) 0 (0/0) 5 (0/5) 1 (1/0) 4 (1/3) 0 (0/0) 3.8 (2.4/1.4)
10 8 (8/0) 0 (0/0) 0 (0/0) 3 (0/3) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1.6 (1.2/0.4)
11 9 (9/0) 0 (0/0) 0 (0/0) 2 (0/2) 0 (0/0) 0 (0/0) 1 (1/0) 7 (0/7) 0 (0/0) 2.8 (1.8/1.0)
12 5 (5/0) 1 (0/1) 2 (0/2) 0 (0/0) 1 (1/0) 0 (0/0) 1 (1/0) 8 (1/7) 0 (0/0) 2.7 (1.6/1.1)
13 4 (4/0) 0 (0/0) 1 (0/1) 2 (0/2) 0 (0/0) 1 (0/1) 0 (0/0) 2 (0/2) 1 (1/0) 1.3 (0.7/0.6)
14 3 (3/0) 0 (0/0) 1 (0/1) 0 (0/0) 0 (0/0) 0 (0/0) 2 (2/0) 0 (0/0) 0 (0/0) 1.1 (1.0/0.1)
15 5 (5/0) 0 (0/0) 1 (1/0) 1 (0/1) 2 (0/2) 1 (0/1) 0 (0/0) 2 (1/1) 1 (1/0) 1.8 (1.2/0.6)
16 2 (2/0) 0 (0/0) 0 (0/0) 2 (0/2) 1 (1/0) 2 (0/2) 0 (0/0) 1 (1/0) 1 (0/1) 1.6 (1.0/0.6)
17 4 (4/0) 0 (0/0) 0 (0/0) 1 (0/1) 1 (1/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 0.9 (0.8/0.1)
18 5 (5/0) 1 (1/0) 0 (0/0) 0 (0/0) 0 (0/0) 3 (3/0) 1 (1/0) 1 (0/1) 0 (0/0) 1.9 (1.8/0.1)
19 1 (1/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (0/1) 0 (0/0) 0 (0/0) 1 (0/1) 0 (0/0) 0.4 (0.2/0.2)
20 5 (5/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 2 (2/0) 1 (1/0) 0 (0/0) 1.2 (1.2/0.0)
21 1 (0/1) 0 (0/0) 0 (0/0) 1 (0/1) 0 (0/0) 1 (0/1) 0 (0/0) 2 (0/2) 0 (0/0) 0.6 (0.0/0.6)
22 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 0 (0/0) 1 (1/0) 0 (0/0) 0.3 (0.3/0.0)
X 28 (27/1) 1 (0/1) 1 (1/0) 7 (5/2) 2 (2/0) 5 (4/1) 1 (1/0) 5 (3/2) 0 (0/0) 7.0 (6.2/0.2)
Y 4 (4/0) 1 (1/0) 0 (0/0) 6 (6/0) 0 (0/0) 14 (14/0) 2 (2/0) 0 (0/0) 0 (0/0) 3.0 (3.0/0.0)
Total 218 (211/7) 12 (8/4) 34 (27/7) 44 (12/32) 55 (51/4) 37 (24/13) 24 (23/1) 89 (15/74) 117 (115/2) 82.7 (66.6/16.1)
  1. 28 % of CNAs (0–97 %) were located on 3q in CD19+ cells compared to 5 % (0–11 %) in CD19 cells (data not shown)