Table 1 Genes regulated in the EBS-DM model keratinocyte cell line KEB7 identified by microarray analysis
Microarray candidate genes (accession number) | Regulation microarray | log2 fold-change microarray | Fold-change qRT-PCR | Significance of qRT-PCR (fold-change set −2) |
|---|---|---|---|---|
KRT19 (NM_002276) | Significant down-regulated | −5.74 | −1633.0 | Significant |
MIR492 (NR_030171) | Significant down-regulated | −4.46 | n.d. | n.d. |
KYNU (NM_003937) | Significant down-regulated | −4.20 | −276.6 | Significant |
H19, MIR675 (NR_030533) | Significant down-regulated | −4.12 | n.d. | n.d. |
EYA4 (NM_004100) | Significant down-regulated | −4.03 | n.d. | n.d. |
PDZK1 (NM_002614) | Significant down-regulated | −3.86 | −8.0 | Significant |
TMPRSS15 (NM_002772) | Significant down-regulated | −3.85 | n.d. | n.d. |
OLFM4 (NM_006418) | Significant down-regulated | −3.73 | −8.4 | Significant |
SLC38A4 (NM_018018) | Significant down-regulated | −3.67 | −12.3 | Significant |
BST2 (NM_004335) | Significant down-regulated | −3.53 | −137.3 | Significant |
ITGBL1 (NM_004791) | Significant down-regulated | −2.99 | n.d. | n.d. |
EDIL3 (NM_005711) | Significant down-regulated | −2.91 | n.d. | n.d. |
PPARGC1A (NM_013261) | Significant down-regulated | −2.79 | −964.3 | Significant |
GALNT5 (NM_014568) | Significant down-regulated | −2.73 | −78.3 | Significant |
CDR1 (NM_004065) | Significant down-regulated | −2.71 | n.d. | n.d. |
FKBP10 (NM_021939) | Significant down-regulated | −2.63 | −26.8 | Significant |
GIPC2 (NM_017655) | Significant down-regulated | −2.55 | −71.4 | Significant |
AMOT (NM_001113490) | Significant down-regulated | −2.53 | −3015.8 | Significant |
ZNF114 (NM_153608) | Significant down-regulated | −2.52 | −10.2 | Significant |
CLEC2B (NM_005127) | Significant down-regulated | −2.50 | −182.1 | Significant |
FAM198B (NM_001031700) | Significant down-regulated | −2.50 | −14.0 | Significant |
SLC2A3 (NM_006931) | Significant down-regulated | −2.48 | −88.7 | Significant |
CAPNS2 (NM_032330) | Significant down-regulated | −2.43 | −9.0 | Significant |
TBX18 (NM_001080508) | Significant down-regulated | −2.43 | −89.4 | Significant |
LRCH2 (NM_020871) | Significant down-regulated | −2.36 | −44.2 | Significant |
NEFM (NM_005382) | Significant down-regulated | −2.34 | −7.7 | Significant |
CPT1C (NM_001136052) | Significant down-regulated | −2.32 | −13.4 | Significant |
ZNF43 (NM_001256648) | Significant down-regulated | −2.22 | −9.7 | Significant |
LY75, LY75-CD302 (NM_002349) | Significant down-regulated | −2.17 | −8.6 | Significant |
LOC100129717, NEFL (NM_006158) | Significant down-regulated | −2.16 | n.d. | n.d. |
GLDC (NM_000170) | Significant down-regulated | −2.14 | −307.6 | Significant |
TMTC1 (NM_001193451) | Significant down-regulated | −2.13 | −6.7 | Significant |
SLCO1B3 (NM_019844) | Significant down-regulated | −2.10 | −988.0 | Significant |
SMOC2 (NM_022138) | Significant down-regulated | −2.09 | n.d. | n.d. |
SLC6A14 (NM_007231) | Significant down-regulated | −2.02 | −4.9 | Significant |
SLC24A3 (NM_020689) | Significant down-regulated | −1.97 | −53.0 | Significant |
EPSTI1 (NM_001002264) | Significant down-regulated | −1.96 | −10.8 | Significant |
SATB2 (NM_001172509) | Significant down-regulated | −1.94 | −224.2 | Significant |
HSD17B2 (NM_002153) | Significant down-regulated | −1.89 | −59.8 | Significant |
GHR (NM_000163) | Significant down-regulated | −1.87 | n.d. | n.d. |
TFPI2 (NM_006528) | Significant down-regulated | −1.86 | n.d. | n.d. |
AKR1B10 (NM_020299) | Significant down-regulated | −1.86 | −3.8 | Significant |
ARHGAP28 (NM_001010000) | Significant down-regulated | −1.81 | n.d. | n.d. |
LOC100506941, NNMT (NM_006169) | Significant down-regulated | −1.81 | n.d. | n.d. |
GPC3 (NM_001164617) | Significant down-regulated | −1.81 | −427.1 | Significant |
IFITM3 (JQ610621) | Significant down-regulated | −1.80 | −8.7 | Significant |
HOXD10 (NM_002148) | Significant down-regulated | −1.79 | −100.8 | Significant |
MSX2 (NM_002449) | Significant down-regulated | −1.77 | −9.5 | Significant |
IL17RB (NM_018725) | Significant down-regulated | −1.75 | −9.1 | Significant |
BLMH (NM_000386) | Significant down-regulated | −1.74 | −4.8 | Significant |
SOX2 (NM_003106) | Significant down-regulated | −1.72 | n.d. | n.d. |
HIST1H | Significant down-regulated | −1.70 | n.d. | n.d. |
SLC9A2 (NM_003048) | Significant down-regulated | −1.69 | −17.0 | Significant |
CPNE1, RBM12 (NM_006047) | Significant down-regulated | −1.68 | −14.9 | Significant |
WDR17 (NM_170710) | Significant down-regulated | −1.60 | −11.2 | Significant |
RB1 (NM_000321) | Significant down-regulated | −1.58 | −4.4 | Significant |
DPYD (NM_000110) | Significant down-regulated | −1.57 | −3.7 | Significant |
PCCA (NM_000282) | Significant down-regulated | −1.56 | n.d. | n.d. |
ZNF570 (NM_001300993) | Significant down-regulated | −1.51 | n.d. | n.d. |
PRTFDC1 (NM_020200) | Significant down-regulated | −1.49 | n.d. | n.d. |
GLRX (NM_002064) | Significant down-regulated | −1.47 | −2.8 | Significant |
SLC7A2 (NM_003046) | Significant down-regulated | −1.45 | −1.5 | Non-significant |
PPP1R16B (NM_015568) | Significant down-regulated | −1.44 | −8.0 | Significant |
IKZF3 (NM_012481) | Significant down-regulated | −1.41 | −1.8 | Non-significant |
GTF2H2D | Significant down-regulated | −1.40 | −4.2 | Significant |
CDK14 (NM_001287135) | Significant down-regulated | −1.40 | n.d. | n.d. |
REPS2 (NM_004726) | Significant down-regulated | −1.38 | −4.5 | Significant |
MEST (NM_002402) | Significant down-regulated | −1.38 | n.d. | n.d. |
CYP4V2 (NM_207352) | Significant down-regulated | −1.35 | −4.7 | Significant |
GPR143 (NM_000273) | Significant down-regulated | −1.35 | −2.1 | Significant |
GTF2H2 (NM_001515) | Significant down-regulated | −1.35 | −4.0 | Significant |
CYP7B1 (NM_004820) | Significant down-regulated | −1.32 | n.d. | n.d. |
BCL11A (NM_022893) | Significant down-regulated | −1.31 | −3.6 | Significant |
MERTK (NM_006343) | Significant down-regulated | −1.30 | −5.0 | Significant |
PRDM5 (NM_018699) | Significant down-regulated | −1.28 | −6.1 | Significant |
ACOXL (NM_001142807) | Significant down-regulated | −1.28 | −11.6 | Significant |
AHCY (NM_000687) | Significant down-regulated | −1.27 | −2.0 | Significant |
ARMCX2 (NM_177949) | Significant down-regulated | −1.24 | −22.5 | Significant |
PAX6 (NM_000280) | Significant down-regulated | −1.23 | −14.2 | Significant |
HOXD11 (NM_021192) | Significant down-regulated | −1.23 | −49.4 | Significant |
SMARCA1 (NM_003069) | Significant down-regulated | −1.22 | −4.1 | Significant |
IFI44L (NM_006820) | Significant down-regulated | −1.22 | −57.0 | Significant |
GALNTL4 | Significant down-regulated | −1.20 | n.d. | n.d. |
PITRM1 (NM_001242307) | Significant down-regulated | −1.19 | −2.3 | Significant |
CRIP2 (NM_001312) | Significant down-regulated | −1.18 | n.d. | n.d. |
NAP1L5 (NM_153757) | Significant down-regulated | −1.15 | −20.7 | Significant |
IPO7 (NM_006391) | Significant down-regulated | −1.15 | n.d. | n.d. |
SAAL1 (NM_138421) | Significant down-regulated | −1.13 | n.d. | n.d. |
KRTCAP3 (NM_001168364) | Significant down-regulated | −1.12 | n.d. | n.d. |
FAM159A (NM_001042693) | Significant down-regulated | −1.11 | n.d. | n.d. |
EYA1 (NM_172060) | Significant down-regulated | −1.09 | n.d. | n.d. |
PIGU (NM_080476) | Significant down-regulated | −1.09 | −3.2 | Significant |
CDC25B (NM_021873) | Significant down-regulated | −1.09 | n.d. | n.d. |
NKX2-6 (NM_001136271) | Significant down-regulated | −1.07 | n.d. | n.d. |
HTATIP2 (NM_001098520) | Significant down-regulated | −1.05 | n.d. | n.d. |
ILK (NM_004517) | Significant down-regulated | −1.05 | n.d. | n.d. |
ACSF2 (NM_001288968) | Significant down-regulated | −1.04 | n.d. | n.d. |
PDZD2 (NM_178140) | Significant down-regulated | −1.03 | n.d. | n.d. |
CENPH (NM_022909) | Significant down-regulated | −1.03 | n.d. | n.d. |
TOX (NM_014729) | Significant down-regulated | −1.02 | n.d. | n.d. |
VSTM2L (NM_080607) | Significant down-regulated | −1.01 | n.d. | n.d. |
SYT17 (NM_016524) | Significant down-regulated | −1.01 | n.d. | n.d. |
Microarray candidate genes (accession number) | Regulation microarray | log2 fold-change microarray | Fold-change qRT-PCR | Significance of qRT-PCR (fold-change set +2) |
|---|---|---|---|---|
TDRD12 (NM_001110822) | Significant up-regulated | 3.98 | 618.1 | Significant |
NEFH (NM_021076) | Significant up-regulated | 3.45 | 1174.9 | Significant |
NLRP2 (NM_017852) | Significant up-regulated | 3.39 | 154.3 | Significant |
TSPYL5 (NM_033512) | Significant up-regulated | 3.20 | n.d. | n.d. |
KLK5 (NM_012427) | Significant up-regulated | 3.18 | 62.8 | Significant |
ENPP1 (NM_006208) | Significant up-regulated | 3.09 | 6.1 | Significant |
GJB6 (NM_001110219) | Significant up-regulated | 2.98 | 24.4 | Significant |
ZFP42 (NM_174900) | Significant up-regulated | 2.84 | 313.7 | Significant |
RNF212 (NM_001131034) | Significant up-regulated | 2.59 | n.d. | n.d. |
DDX43 (NM_018665) | Significant up-regulated | 2.51 | n.d. | n.d. |
DKK1 (NM_012242) | Significant up-regulated | 2.40 | 45.3 | Significant |
CYYR1 (NM_052954) | Significant up-regulated | 2.36 | 19.6 | Significant |
C10orf99 (NM_207373) | Significant up-regulated | 2.33 | 10.6 | Significant |
SYCP2 (NM_014258) | Significant up-regulated | 2.23 | 10.2 | Significant |
ZNF136 (NM_003437) | Significant up-regulated | 2.17 | n.d. | n.d. |
CCDC144A (NM_014695) | Significant up-regulated | 2.11 | n.d. | n.d. |
PRICKLE1 (NM_153026) | Significant up-regulated | 2.08 | 52.4 | Significant |
SLC44A5 (NM_152697) | Significant up-regulated | 2.06 | 100.5 | Significant |
PLA2G7 (NM_005084) | Significant up-regulated | 2.05 | 18.6 | Significant |
CCDC144A, CCDC144B, CCDC144C | Significant up-regulated | 2.01 | n.d. | n.d. |
MOXD1 (NM_015529) | Significant up-regulated | 1.97 | 15.9 | Significant |
ZNF700 (NM_144566) | Significant up-regulated | 1.96 | n.d. | n.d. |
WNT5A (NM_003392) | Significant up-regulated | 1.94 | 6.9 | Significant |
WISP3 (CCN6) (NM_003880) | Significant up-regulated | 1.92 | 103.8 | Significant |
ARHGEF9 (NM_015185) | Significant up-regulated | 1.88 | 25.0 | Significant |
HSD17B11 (NM_016245) | Significant up-regulated | 1.87 | 3.1 | Significant |
BGN (NM_001711) | Significant up-regulated | 1.87 | 1.8 | Non-significant |
ADAMTSL3 (NM_207517) | Significant up-regulated | 1.84 | 26.4 | Significant |
FAM102B (NM_001010883) | Significant up-regulated | 1.78 | 2.5 | Significant |
SGMS1 (NM_147156) | Significant up-regulated | 1.76 | 2.9 | Significant |
ARHGAP29 (NM_004815) | Significant up-regulated | 1.75 | 4.1 | Significant |
H2AFY2 (NM_018649) | Significant up-regulated | 1.74 | 1.8 | Non-significant |
SLC15A2 (NM_021082) | Significant up-regulated | 1.73 | 4.6 | Significant |
ROBO1 (NM_002941) | Significant up-regulated | 1.73 | 5.6 | Significant |
ERCC6, PGBD3 (NM_000124) | Significant up-regulated | 1.68 | 6.0 | Significant |
NREP (NM_004772) | Significant up-regulated | 1.67 | 3.0 | Significant |
KIAA1324L (NM_001142749) | Significant up-regulated | 1.62 | 149.8 | Significant |
SNORD116-21 (NR_003335) | Significant up-regulated | 1.62 | n.d. | n.d. |
HOXA9 (NM_152739) | Significant up-regulated | 1.59 | 1.3 | Non-significant |
ROR1 (NM_005012) | Significant up-regulated | 1.59 | 9.5 | Significant |
RPS23 (NM_001025) | Significant up-regulated | 1.58 | n.d. | n.d. |
MAN1A1 (NM_005907) | Significant up-regulated | 1.55 | 1.0 | Non-significant |
STOX1 (NM_152709) | Significant up-regulated | 1.54 | −1.1 | Non-significant |
RPL10, SNORA70 (NM_006013) | Significant up-regulated | 1.53 | −1.1 | Non-significant |
CCDC144C | Significant up-regulated | 1.52 | n.d. | n.d. |
ZFAND4 (NM_174890) | Significant up-regulated | 1.50 | 2.5 | Significant |
NOX5, SPESP1 (NM_024505) | Significant up-regulated | 1.50 | n.d. | n.d. |
EVC2 (NM_147127) | Significant up-regulated | 1.46 | n.d. | n.d. |
SELENBP1 (NM_003944) | Significant up-regulated | 1.45 | 64.8 | Significant |
ZNF334 (NM_018102) | Significant up-regulated | 1.43 | 15.6 | Significant |
PTPN20C (NM_001042357) | Significant up-regulated | 1.43 | n.d. | n.d. |
IRX4 (NM_001278632) | Significant up-regulated | 1.41 | 90.4 | Significant |
PTPN20A (NM_001042357) | Significant up-regulated | 1.41 | 29.5 | Significant |
PTPN20B (NM_001042357) | Significant up-regulated | 1.40 | n.d. | n.d. |
PNMAL1 (NM_018215) | Significant up-regulated | 1.40 | 2.9 | Significant |
NID1 (NM_002508) | Significant up-regulated | 1.38 | 1.8 | Non-significant |
ZNF32 (NM_006973) | Significant up-regulated | 1.37 | 3.5 | Significant |
ASAH2 (NM_019893) | Significant up-regulated | 1.37 | n.d. | n.d. |
FLJ20444, LOC554249 | Significant up-regulated | 1.36 | n.d. | n.d. |
TP53INP1 (NM_033285) | Significant up-regulated | 1.35 | 1.6 | Non-significant |
SLC16A4 (NM_004696) | Significant up-regulated | 1.34 | 16.4 | Significant |
FRG1B (NR_003579) | Significant up-regulated | 1.32 | n.d. | n.d. |
AHI1 (NM_001134831) | Significant up-regulated | 1.32 | 3.7 | Significant |
MSLN (NM_005823) | Significant up-regulated | 1.30 | n.d. | n.d. |
ELAVL2 (NM_004432) | Significant up-regulated | 1.29 | 4.3 | Significant |
SLC16A9 (NM_194298) | Significant up-regulated | 1.28 | 2.4 | Significant |
FAM25A, FAM25B, FAM25C, FAM25G (NM_001146157) | Significant up-regulated | 1.28 | 2.5 | Significant |
MAPK8 (NM_002750) | Significant up-regulated | 1.28 | 2.2 | Significant |
CDC14B (NM_003671) | Significant up-regulated | 1.26 | 3.0 | Significant |
GABPB2 (NM_144618) | Significant up-regulated | 1.26 | 2.3 | Significant |
DENND1B (NM_144977) | Significant up-regulated | 1.25 | n.d. | n.d. |
TCHH (NM_007113) | Significant up-regulated | 1.23 | 28.0 | Significant |
LMF1 (NM_022773) | Significant up-regulated | 1.22 | 5.1 | Significant |
CSTF2T (NM_015235) | Significant up-regulated | 1.22 | 2.2 | Significant |
IFFO2 (NM_001136265) | Significant up-regulated | 1.21 | n.d. | n.d. |
SGK1 (NM_005627) | Significant up-regulated | 1.19 | 4.2 | Significant |
HOXC10 (NM_017409) | Significant up-regulated | 1.19 | 2.2 | Significant |
STRBP (NM_018387) | Significant up-regulated | 1.18 | n.d. | n.d. |
UAP1 (NM_003115) | Significant up-regulated | 1.18 | 2.3 | Significant |
POPDC2 (NM_022135) | Significant up-regulated | 1.18 | 3.9 | Significant |
DSEL (NM_032160) | Significant up-regulated | 1.17 | −1.2 | Non-significant |
AOX1 (NM_001159) | Significant up-regulated | 1.16 | 1.4 | Non-significant |
ZNF502 (NM_033210) | Significant up-regulated | 1.16 | 500.9 | Significant |
LRP12 (NM_013437) | Significant up-regulated | 1.15 | n.d. | n.d. |
ADHFE1 (NM_144650) | Significant up-regulated | 1.12 | 1.8 | Non-significant |
FAM21D | Significant up-regulated | 1.11 | n.d. | n.d. |
SNORD64 (NR_001294) | Significant up-regulated | 1.10 | n.d. | n.d. |
FAM21A, FAM21B, FAM21C | Significant up-regulated | 1.09 | n.d. | n.d. |
EFEMP1 (NM_001039348) | Significant up-regulated | 1.09 | n.d. | n.d. |
KGFLP1, KGFLP2 | Significant up-regulated | 1.08 | n.d. | n.d. |
ANKRD2 (NM_020349) | Significant up-regulated | 1.07 | −1.4 | Non-significant |
PIK3R3 (NM_003629) | Significant up-regulated | 1.07 | n.d. | n.d. |
HS3ST3B1 (NM_006041) | Significant up-regulated | 1.07 | 1.2 | Non-significant |
PTPN20A, PTPN20B (NM_001042357) | Significant up-regulated | 1.07 | n.d. | n.d. |
FAM21A, FAM21B, FAM21C | Significant up-regulated | 1.07 | n.d. | n.d. |
APBB2 (NM_004307) | Significant up-regulated | 1.06 | n.d. | n.d. |
DUOX1 (NM_017434) | Significant up-regulated | 1.06 | n.d. | n.d. |
ZNF627 (NM_145295) | Significant up-regulated | 1.06 | n.d. | n.d. |
MCOLN2 (NM_153259) | Significant up-regulated | 1.06 | n.d. | n.d. |
APOB (NM_000384) | Significant up-regulated | 1.04 | n.d. | n.d. |
AJAP1 (NM_018836) | Significant up-regulated | 1.04 | n.d. | n.d. |
BNC2 (NM_017637) | Significant up-regulated | 1.03 | n.d. | n.d. |
LOC100652860, LOC100653093, TTC6 | Significant up-regulated | 1.03 | n.d. | n.d. |
FKTN (NM_001079802) | Significant up-regulated | 1.03 | n.d. | n.d. |
TMEM204 (NM_024600) | Significant up-regulated | 1.02 | n.d. | n.d. |
BMS1P1, BMS1P5 (NR_003611) | Significant up-regulated | 1.00 | n.d. | n.d. |