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Table 1 Methylglyoxal-dependent modifications of catalase

From: Arg354 in the catalytic centre of bovine liver catalase is protected from methylglyoxal-mediated glycation

Sequence

Position

Modification

m/z

Peak area

% modified

F.DRERIPERVVH.A

65–75

nm

469.260

141,239,376

 

MG-H1

487.263

1,972,739

1.4

DHI

493.267

2,816,325

1.9

F.GYFEVTHDITRY.S

83–94

nm

750.860

35,923,631

 

MG-H1

777.865

6,243,848

13.2

DHI

786.870

5,107,276

10.8

F.SDRGIPDGHRHM.N

201–212

nm

459.886

36,013,455

 

MG-H1

477.889

1,015,794

2.7

DHI

483.893

1,049,274

1.0

L.AHEDPDYGLRDLF.N

254–266

nm

774.360

104,144,627

 

MG-H1

801.365

140,068

0.1

DHI

810.370

393,409

0.4

M.LQGRLF.A

351–356

nm

367.221

68,678,448

 

MG-H1

394.226

nd

0.0

DHI

403.231

nd

0.0

H.RHRLGPNYLQIPVNCPY.R

363–379

nm

699.698

13,777,078

 

MG-H1

717.702

225,543

1.6

DHI

723.705

130,193

0.9

F.NSANDDNVTQVRTF.Y

433–446

nm

790.868

194,414,574

 

MG-H1

817.874

14,193,861

6.3

DHI

826.879

16,307,423

7.3

  1. Arginine residues of interest are printed in italic letters
  2. m/z mass to charge ratio, nm non-modified catalase peptide ion, nd not detected