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Table 2 Results from molecular identification of greater sage-grouse nest and carcass predators using both mitochondrial and microsatellite DNAs

From: Molecular forensics in avian conservation: a DNA-based approach for identifying mammalian predators of ground-nesting birds and eggs

Nest (N) or carcass (C) ID # Eggs # Successful sequences # msat genotypes Sequence ID from BLAST STRUCTURE assignment Molecular ID Field cues Camera traps
CB CR Set A Set B
N1 7 2     99 % cow   Cow Bird or Bobcat Indeterminate
N2 3 1     98 % coyote   Coyote Cow Cow
N3 4 2     99 % coyote   Coyote Coyote Coyote
N4 7 1 2 4 1 99 % coyote 97 % coyote Coyote Coyote Coyote
N5 7 1 3 1   99 % coyote 97 % coyote Coyote Coyote Coyote
N6 1        Indeterminate Indeterminate Raven
N7 3 3   1 1 99 % coyote/99 % wolf (WI) 38 % coyote/37 % wolf (WY)/17 % wolf (WI) Wild canid Raven or coyote Indeterminate
N8 8 8 1 1   99 % coyote/99 % wolf (WI) 98 % coyote Coyote Magpie or coyote Coyote
N9 1   1    99 % dog   Dog Raven or snake Indeterminate
N10 1 1     97 % striped skunk   Striped skunk Indeterminate Striped skunk or badger
N11 8   3 1   100 % dog/99 % deer mouse 92 % coyote Coyote/dog/deer mouse Indeterminate Indeterminate
N12 1   1    dog   Dog Indeterminate Weasel
N13 3        Indeterminate Indeterminate Indeterminate
N14 6        Indeterminate Indeterminate Indeterminate
        Percent nests with molecular ID: 78.6 %   
C1 NA        Indeterminate Indeterminate NA
C2 NA Y   1 1 100 % coyote/100 % wolf (WI) 96 % coyote Coyote Coyote NA
C3 NA Y   1 1 98 % coyote/98 % wolf (WI) 76 % coyote/20 % dog Canid Indeterminate NA
C4 NA        Indeterminate Indeterminate NA
C5 NA Y    1 99 % coyote/99 % wolf (WI)   Wild canid Indeterminate NA
C6 NA        Indeterminate Indeterminate NA
C7 NA        Indeterminate Indeterminate NA
        Percent carcasses with molecular ID: 42.9 %   
  1. Listed in the table are ID for each depredated carcass or nest, the total number of eggs collected from each nest (# eggs), the number of eggs with successful predator ID using DNA sequences (# successful sequences; CB cytochrome b, CR control region), # of microsatellite genotypes used for species idenitifcation (# msat genotypes for set A and set B), species identification using genotypes and assigned in STRUCTURE (STRUCTURE assignment), identification of predator based on sequences, microsatellites, or both (molecular ID), predator identification in the field from nest remains (Field cue), and predator identification based on trails cameras placed on nest. The percent match was generated from BLAST. WY Wyoming and WI Wisconsin
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BMC Research Notes

ISSN: 1756-0500

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