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Fig. 4 | BMC Research Notes

Fig. 4

From: Buserelin treatment to rats causes enteric neurodegeneration with moderate effects on CRF-immunoreactive neurons and Enterobacteriaceae in colon, and in acetylcholine-mediated permeability in ileum

Fig. 4

The colonic microbiota was analyzed with next generation sequencing using the ion torrent platform. Firmicutes and Bacteroidetes were the two most dominant phyla in both controls (C) and buserelin (B)-treated rats. There were no significant differences between the C and B group regarding the relative abundance of any phyla or families (a). PCR-quantified amounts of selected bacterial families in tissue from ileum (b) and colon (c) of controls and buserelin-treated rats. The amounts of 16S rRNA gene copies from total bacteria and the C. leptum group were not statistically different between the C and B group in neither ileum nor colon. The amount of 16S rRNA gene copies from Enterobacteriaceae was significantly lower in tissue from colon (p = 0.020), but not ileum, in buserelin-treated rats compared with control rats. Samples under the detection limit were set to the highest possible detection limit. C = 7 rats and B = 11 rats. Results are presented as medians and interquartile ranges and were analyzed by the Mann–Whitney U-test. Statistical significance is indicated by *p < 0.05

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BMC Research Notes

ISSN: 1756-0500

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