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Fig. 1 | BMC Research Notes

Fig. 1

From: Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR

Fig. 1

Schematic representation of SCCmec excision and formation of its circular form. The figure shows the linear SCCmec integrated in the chromosome (a), the circularized SCCmec excised from the chromosome (b) and the religated chromosome after excision (c). Chromosomal DNA is shown in yellow and SCCmec DNA is shown in dark blue. Locations of primers used for qPCR are shown by arrows and their amplicons are highlighted in light blue. qControl amplicon is used to determine the number of chromosomes in the assay, while qEx and qCirc specifically detect excisants and circularized SCCmec, respectively

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BMC Research Notes

ISSN: 1756-0500

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