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Table 1 Bacterial strains and plasmids

From: Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR

Strain or plasmid Relevant characteristics References
Strains
E. coli DH5α Host for DNA cloning Laboratory collection
S. aureus
 RN4220 Restriction-deficient derivative of S. aureus RN450 [11]
 N315 MRSA carrying type II SCCmec [12]
 N315EX Isogenic MSSA derivative of N315 This study
Plasmids
pUC28 ColE1 replicon, high copy number vector for cloning, ampicillin resistance [13]
pSR3-1 Thermosensitive-replicon plasmid carrying the ccrAB genes of strain N315 (used for SCCmec excision in N315), tetracycline resistance [7]
qPlasmid Plasmid used for qPCR analysis This study