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Fig. 6 | BMC Research Notes

Fig. 6

From: Optimization of canine interleukin-12 production using a baculovirus insect cell expression system

Fig. 6

Assessment of IFN-γ production by peripheral blood mononuclear cells (PBMC) stimulated with rsccaIL-12L. PBMC from six adult mongrel dogs were cultured for 48 h with: complete RPMI 1640 medium alone (medium), COS-7 cells supernatant containing canine IL-2 at 20 % (IL-2), purified rsccaIL-12L at 20 ng/mL (IL-12); or purified sccaIL-12L at 20 ng/mL combined with COS-7 cell SN-caIL-2 (IL-2 + IL-12). IFN-γ was assessed in the PBMC supernatants by capture ELISA. Data represent the average of duplicates and means obtained from six animals. Repeated measures ANOVA: p < 0.0001, Dunnett´s test *p < 0.05

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