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Table 2 Characteristics of 16 SSR loci in Lepidonotothen nudifrons and two additional congeneric species

From: Microsatellite markers for the notothenioid fish Lepidonotothen nudifrons and two congeneric species

Locus name

Accession #

Blastx Top Hit

Repeat content

Primers (5′–3′)

Fluorescent label

Multiplex mix

Ln

Ls

Ll

Ln_22268

HACN01006381

(GA)9

F: TGGATGGTTTTTCGTTTGACCAA

R: TGAAGAGCTTTGGAGCAACAA

TAMRA

1

M

M

M

Ln_22517

HACN01006568

(AT)9

F: TGATTCATCCTCTGGGTACCAT

R: AGCTCGTAGCTATTCCAGCA

HEX

2

P

M

Ln_23194

HACN01007070

(TA)9

F: TCCGGAGGCTGCGTTCAGAG

R: CTGCTTGAGGACCGGCTCAG

HEX

1

M

P

P

Ln_32298

HACN01014077

Dolichol-phosphate mannosyltransferase subunit 3 (Q3ZC71)

(AC)9

F: AAAGTAGTCCGTGCATCCCTT

R: GTCTTACGACCTGTTGGAGC

TAMRA

2

M

M

M

Ln_34878

HACN01015998

(AG)10

F: GTTGCAGTCACGTTGAAAGC

R: ACGAATCCAAACCTTGCAACAT

FAM

3

P

Ln_35217

HACN01016251

(CT)9

F: CCACTACACAGCTGAAATGGT

R: GGCATGATTGGAGCACTTCC

HEX

1

P

P

P

Ln_36100

HACN01016925

(AG)9

F: TAAAGCTCCAGCCGATACTGG

R: TGGGAATTGAACAGATGTCACC

TAMRA

1

P

M

P

Ln_36156

HACN01016972

(GT)9

F: GTCTGCTCATGTCCAACACAT

R: AGGTGTTTCTGTAAACCCTCG

FAM

2

P

P

P

Ln_40551

HACN01020461

(TA)9

F: TAACTGATGCATGCCCAGGAACTT

R: GCTTCCTAAAAGCTCCACAGAT

FAM

1

P

P

P

Ln_41246

HACN01021014

(AT)9

F: GCTTAAATCCAACCATAGACGCA

R: CTCATCAGGACTTCACGTGC

FAM

2

M

Ln_41281

HACN01021039

Uncharacterized protein C1orf21 homolog (Q8K207)

(GT)9

F: TGTGTTTTTGGACGTATGGCA

R: GCTTCCATTTTGGGAGTACCT

HEX

1

P

P

P

Ln_42016

HACN01021779

(GT)9

F: ACATCACTTGCAAATAGGCCGGTG

R: CCGTCATGCACCTGGGAGTT

HEX

3

P

P

P

Ln_42233

HACN01021981

Serine/threonine-protein kinase N2 (A7MBL8)

(AT)9

F: ATTCCTGACTGCCAAAGACG

R: TGTTGACTTGCAGACTGAACG

FAM

1

P

M

P

Ln_42701

HACN01022446

Hepatic leukemia factor (Q8BW74)

(AT)9

F: TGGACTTCACAAGCGTGGCT

R: ATTGAAAGTGTGCATAGTCCGT

FAM

1

M

M

M

Ln_45257

HACN01024934

(TG)9

F: AAGACCAGGGCGAGTCTGAC

R: CTCTAACCAGAACACCGCCT

HEX

2

P

P

P

Ln_45589

HACN01025236

(AG)9

F: CGTGTCTAGGAGCTACAGCA

R: CCAGGTTGGTGGCATCTCTT

TAMRA

2

P

P

P

      

Total

11

8

10

  1. The table reports: the name of each locus (Ln = Lepidonotothen nudifrons and the transcript number), Accession number of the de novo assembled transcripts of L. nudifrons (European Nucleotide Archive -ENA- project number PRJEB8919) [13], the putatively annotated loci (Blastx Top Hit, e-value < 10−9), the repeat content, the forward (F) and reverse (R) primer sequences, the fluorescent label, the multiplex pool (mix). Variability, expressed in terms (P) polymorphic, (M) monomorphic or (-) unsuccessful locus, was assessed on 21 L. nudifrons (Ln), 20 L. squamifrons (Ls) and 18 L. larseni (Ll) individuals. Total number of polymorphic loci is reported in the last row