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Table 3 Relative assembly scores using different assemblers

From: Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly

Quality metric

Assembler

Genome

Bce

Efa

Sen

Pst

Corrected NG50

SPAdes

1

1

1

1

ABySS

0.69

0.87

0.82

0.70

Velvet

0.71

0.71

0.77

0.94

Edena

0.63

0.72

0.63

0.73

max**

448,776

381,370

292,477

212,702

NGA50

SPAdes

1

1

0.98

1

ABySS

0.93

0.97

1

0.92

Velvet

0.58

0.82

0.92

0.96

Edena

0.40

0.66

0.50

0.66

max**

733,293

416,896

405,154

238,037

  1. A maximal relative assembly score of 1 refers to the assembler with the best assembly performance. All other assembly scores are expressed as relative fractions of their corresponding maximum. For genomes Bce, Efa and Pst each relative assembly score was calculated on the basis of 30 assembly sets. Assembly sets originated from same-genome sequencing libraries differing in category (average insert size), read length and sequencing depth. For Sen the number of assembly sets per relative assembly score was 10
  2. max** Absolute value in nucleotides of the maximal relative assembly score of the column