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Table 2 Prescribed PCR amplification program

From: The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

Mix Steps Temperature °C Duration Cycles
ABI UDG activation 50 2 min Hold
Activation 95 2 min Hold
Denature 95 15 s 40
Anneal/extend 60 60 s
Melt curve analysis 95 10 s  
  60 60 s  
  97 5 s  
BioRad Activation 95 3 min Hold
Denature 95 15 s 40
Anneal/extend 60 45 s
Melt curve analysis 95 10 s  
  55 60 s  
  95 15 s  
Roche Activation 95 10 min Hold
Denature 95 10 s 40
Anneal 60 10 s
Extend 72 10 s
Melt curve analysis 95 10 s  
  65 60 s  
  95 15 s  
General lab PCR program Activation 95 10 min Hold
Denature 95 15 s 45
Anneal/extend 60 60 s
Melt curve analysis 95 10 s  
  55 60 s  
  97 5 s  
  1. The ramp of each machine were set to default