Fig. 1
From: Lentiviral gene transfer into human and murine hematopoietic stem cells: size matters
Schematic representation of the elements cloned into our Gateway compatible LeGO-DEST vector. The different elements and their sizes (bp) that were cloned into our Gateway compatible LeGO-DEST vector to generate lentiviral expression vectors of different sizes between SIN-LTRs: 4215, 5190 and 5750 bp. SFFV: Spleen Focus Forming Virus promoter, T2A: Thosea asigna virus 2A element, BFP: blue fluorescent protein. Grey boxes indicate lentiviral elements in LeGO-DEST