Establishment of anti-mesothelioma monoclonal antibodies

BMC Research Notes

Table 1 Antibodies used in this study and immunocytochemical reactivity

Antibody	Clone	Source	Dilution	Specimen	Case 1	Case 2	Case 3
JMAM1		Our labolatory	Undiluted cell supernatant	Cell smear	+	+	+
JMAM2		Our labolatory	Undiluted cell supernatant	Cell smear	+	+	+
JMAM3		Our labolatory	Undiluted cell supernatant	Cell smear	+	+	+
JMAM4		Our labolatory	Undiluted cell supernatant	Cell smear	+	+	+
WT-1	6F-H2	DAKO	1:200	Cell block	+	±	+
Carletnin	Dak-Calret 1	DAKO	1:100	Cell block	+	+	+
Mesothelin	22A31	IBL	1:1000	Cell block	+	+	+
Podoplanin	D2–40	DAKO	1:200	Cell block	+	+	+
CK5/6	D5/16B4	DAKO	1:50	Cell block	+	+	+
EMA	E29	DAKO	1:100	Cell block	+	+	+
GLUT-1	5B12.3	IBL	1:1000	Cell block	+	+	+
CEA	II-7	DAKO	1:50	Cell block	−	−	−
TTF-1	8G7G3/1	DAKO	1:100	Cell block	−	−	−
Epithelial-related antigen	Moc-31	DAKO	1:100	Cell block	−	−	−

ISSN: 1756-0500