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Table 1 Recombination frequencies of P. aeruginosa PAO1 transformation using linearized plasmid DNA

From: Creation of stable Pseudomonas aeruginosa promoter–reporter fusion mutants using linear plasmid DNA transformation

Constructsa # Of transformants # Of correct recombinants Correct ratio (%)b
pDONR-NT0 8 4 50
pDONR-NT3841P 12 6 50
pDONR-NT45P 7 3 42.8
pDONR-NT3194P 10 5 50
pDONR-ASV0 7 3 42.8
pDONR-ASV3841P 10 4 40
pDONR-ASV45P 11 4 36.4
pDONR-ASV3194P 12 6 50
pDONR-ASV4228P 7 3 42.8
  1. a NT long half-life version of GFP. ASV short half-life (110 min) version of GFP. 0 no promoter element. P promoter element from that particular ORF (e.g. 3841P—promoter from ORF PA3841)
  2. b Ratio # of correct recombinants per # of transformants