Creation of stable Pseudomonas aeruginosa promoter–reporter fusion mutants using linear plasmid DNA transformation

Table 1 Recombination frequencies of P. aeruginosa PAO1 transformation using linearized plasmid DNA

Constructs^a	# Of transformants	# Of correct recombinants	Correct ratio (%)^b
pDONR-NT0	8	4	50
pDONR-NT3841P	12	6	50
pDONR-NT45P	7	3	42.8
pDONR-NT3194P	10	5	50
pDONR-ASV0	7	3	42.8
pDONR-ASV3841P	10	4	40
pDONR-ASV45P	11	4	36.4
pDONR-ASV3194P	12	6	50
pDONR-ASV4228P	7	3	42.8

^a NT long half-life version of GFP. ASV short half-life (110 min) version of GFP. 0 no promoter element. P promoter element from that particular ORF (e.g. 3841P—promoter from ORF PA3841)
^b Ratio # of correct recombinants per # of transformants

ISSN: 1756-0500