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Table 2 Validation of the modified antimicrobial susceptibility testing assay employing the DNA binding fluoroprobe Syto9

From: Assessment of free fatty acids and cholesteryl esters delivered in liposomes as novel class of antibiotic

Strainsa Antibiotic MIC (μg/mL)b
Expectedc Measured
  Standard assay Modified assay
S. aureus ATCC 29213 Oxacillin 0.12–0.5 0.0625 0.5
Cefotaxime 1–4 1–2 2
Tetracycline 0.12–1 0.125–0.25 0.5
Ciprofloxacin 0.12–0.5 0.25–0.5 0.5
E. faecalis ATCC 29212 Oxacillin 8–32 0.5–2 8–16
Tetracycline 8–32 16 16
Ciprofloxacin 0.25–1 0.5–1 2
E. coli ATCC 25922 Cefotaxime 0.03–0.12 0.125 0.125
Tetracycline 0.5–2 1 2–4
Ciprofloxacin 0.004–0.015 0.0156 0.0156
P. aeruginosa ATCC 27853 Cefotaxime 8–32 8 16
Tetracycline 8–32 16 16–32
Ciprofloxacin 0.25–1 0.25 0.5–1
  1. aDesignated ATCC quality control strains for antimicrobial susceptibility testing
  2. bShown are the values derived from two independent experiments each conducted in triplicate
  3. cAccording to Clinical and Laboratory Standards Institute M100-S22 for broth microdilution assay