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Table 3 Raw Cq-value and CVs (coefficients of variation) for adult blood samples

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol CDKN1A FOS IL1B IL8 MYC TP53
MagMax semiautomated
 Average 30.5 26.9 28.4 30.4 29.1 28.3
 SD 0.7 0.2 0.8 0.3 0.4 0.4
 % CV 2.1 0.8 3.0 1.1 1.3 1.3
MagMax manual
 Average 30.1 26.5 27.9 30.0 28.8 27.8
 SD 0.7 0.3 0.7 0.3 0.4 0.3
 % CV 2.5 1.2 2.6 1.1 1.2 1.0
Norgen
 Average 30.7 26.6 28.2 30.2 29.9 28.7
 SD 0.7 0.4 0.8 0.4 0.4 0.4
 % CV 2.3 1.4 2.7 1.2 1.3 1.3
Tempus spin
 Average 29.4 26.1 27.9 29.5 28.9 27.8
 SD 0.7 0.3 0.8 0.4 0.3 0.4
 % CV 2.4 1.1 2.8 1.3 1.2 1.4
Tempus 6-port
 Average 29.9 26.4 28.0 29.9 29.1 28.0
 SD 0.8 0.2 0.7 0.3 0.3 0.3
 % CV 2.5 0.8 2.4 1.0 1.1 1.0
Overall average
 Average 30.1 26.5 28.1 30.0 29.2 28.1
 SD 0.9 0.4 0.8 0.5 0.5 0.5
 % CV 2.8 1.5 2.8 1.5 1.8 1.7
  1. CVs are shown in italics; the overall averaged CVs range between 1.5 and 2.8 %