Immunoassay of S-adenosylmethionine and S-adenosylhomocysteine: the methylation index as a biomarker for disease and health status

Table 1 OD450 measured using different amounts of coating antigens with antibody 84-3

SAM standard (nM)	0.2 µg/mL			PLL-aza-SAM 0.15 µg/mL			0.1 µg/mL
Blank	0.0498	0.056	0.0522	0.0476	0.0471	0.0482	0.0754	0.0515	0.0476
0	1.4709	1.4344	1.4072	1.215	1.2484	1.2651	1.0844	1.1231	1.0633
6.3	1.365	1.3257	1.2673	1.0789	1.0734	1.0842	0.879	0.9122	0.8995
25	1.1769	1.1498	1.1015	0.8231	0.844	0.8334	0.6209	0.6561	0.6679
50	1.0230	0.9856	0.9577	0.6428	0.6362	0.6005	0.4517	0.4555	0.5187
100	0.7687	0.7532	0.7292	0.4292	0.4203	0.4427	0.3036	0.3103	0.3071
Sensitivity^a	3.0 nM			1.7 nM			1.6 nM

^aSensitivity was estimated by the corresponding concentration calculated from standard curve equation given the OD450 (=OD450 at zero antigen − 2 × standard deviation of blank wells). ELISA was performed in triplicates. Mouse anti-SAM antibody 84-3 was used at 1:35,000

ISSN: 1756-0500