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Table 3 SAH standard curve and sensitivity of antibody 301-1

From: Immunoassay of S-adenosylmethionine and S-adenosylhomocysteine: the methylation index as a biomarker for disease and health status

SAH (nM)

OD450

OD450

OD450

Mean

SD

250

0.4116

0.4426

0.442

0.4321

0.0177

125

0.5421

0.6358

0.5372

0.5717

0.0555

62.5

0.6055

0.7115

0.6444

0.6538

0.0536

31.3

0.7285

0.8199

0.7545

0.7676

0.0471

15.6

0.8045

0.8273

0.7975

0.8098

0.0156

7.8

0.7602

0.8113

0.7708

0.7808

0.0270

3.9

0.7722

0.9144

0.7786

0.8217

0.0803

0

0.8770

0.8992

0.8329

0.8697

0.0337

Sensitivitya

~15.6 nM

  1. aSensitivity was estimated by the corresponding concentration calculated from standard curve given the OD 450 (=OD450 at zero antigen – 2 × standard deviation of blank wells). ELISA was performed in triplicates. BSA-SAH was coated at 0.5 µg/mL and mouse anti-SAH antibody 301-1 was used at 1:2000
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BMC Research Notes

ISSN: 1756-0500

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