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Table 2 Aldehyde dehydrogenase levels in whole homogenates of earthworm, viz., M. posthuma, exposed to different heavy metal treatments for three consecutive weeks

From: Aldehyde dehydrogenase expression in Metaphire posthuma as a bioindicator to monitor heavy metal pollution in soil

Treatment

Concentration (ppm)

ALDH activity (mIU/mg protein)

Week 1

Week 2

Week 3

None (control)

Zero

119.7 ± 5.02

62.3 ± 3.3

85.3 ± 3.7

Lead

5

240.6 ± 4.4

832.6 ± 12.1

944.5 ± 5.9

10

172.2 ± 5.9

305.7 ± 8.6

696.3 ± 8.4

15

146.8 ± 3.5

268.4 ± 8.3

595.5 ± 8.9

20

117.2 ± 8.4

237.3 ± 7.5

265.2 ± 3.1

Cadmium

5

452.5 ± 10.6

664.8 ± 11.9

1871.7 ± 6.7

10

314.4 ± 6.5

498.6 ± 10.8

1132.7 ± 12.6

15

288.7 ± 12.4

475.3 ± 7.8

563.5 ± 8.6

20

218.2 ± 3.1

184.2 ± 5.1

348.8 ± 3.7

  1. *  Values represent mean specific activity of ALDH (mIU/mg protein) ± SD; n = 3. Earthworm tissue homogenate (prepared as described in methods section) was used to measure the ALDH activity using acetaldehyde (4 mM) as substrate and NAD (4 mM) as cofactor