Table 2 Descriptions of SSR markers developed for Paspalum plicatulum
Locus name | Source library | GenBank accession number | Reapeat Motif | Ta (C°)1 | Primer Sequence (5′-3′) | Size Range (bp) | P. plicatulum | Plicatula speciesa | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
NA2 | PIC3 | DP4 | NA2 | PIC3 | |||||||
Pp-UNICAMP01 | Lb-1 | KR611535 | (TG)8 | 60 | F: GTGCAACACTATGACACCAG | 173–181 | 4 | 0.64 | 0.70 | 5 | 0.70 |
R: ACAGTGCCCAATTGTTGT | |||||||||||
Pp-UNICAMP02 | Lb-1 | KR611536 | (CGCAC)3 | 51 | F: CTCCACCAACGCCTTAC | 187–203 | 6 | 0.72 | 0.81 | 4 | 0.45 |
R: TAGTCCATACCCTTTCGTTT | |||||||||||
Pp-UNICAMP03 | Lb-1 | KR611537 | (AC)8 | 60 | F: TCTGCTAAGTTACCGCTCAT | 127–167 | 7 | 0.73 | 0.87 | 6 | 0.65 |
R: ATGGATATGGAACTTGATGG | |||||||||||
Pp-UNICAMP04 | Lb-1 | KR611538 | (CA)7 | 60 | F: TTGGATGCACACCAGTATAG | 133–151 | 3 | 0.41 | 0.69 | 7 | 0.66 |
R: CCCTCTTCATTCTCATTCAG | |||||||||||
Pp-UNICAMP05b | Lb-1 | KR611539 | (GT)7 | 60 | F: ATGGATATGGAACTTGATGG | 157–173 | 5 | 0.61 | 0.79 | 6 | 0.77 |
R: CTACGGTCTGCTAAGTCACC | |||||||||||
Pp-UNICAMP06 | Lb-1 | KR611540 | (TC)8 | 51 | F: GGTCCTGGTTGATTGATCT | 155–169 | 7 | 0.73 | 0.88 | 8 | 0.75 |
R: CGGAGTTGAAGATGGACTC | |||||||||||
Pp-UNICAMP07 | Lb-1 | KR611541 | (TCT)4 | 65 | F: AGCCTTGCCTCCAGTC | 222–258 | 6 | 0.66 | 0.74 | 6 | 0.69 |
R: TTTCAGGTTACAGGTTGAGAG | |||||||||||
Pp-UNICAMP08 | Lb-1 | KR611542 | (GT)7 | 51 | F: TGGGTTTGGGATAAGGATAG | 144–170 | 12 | 0.66 | 1.00 | 14 | 0.69 |
R: GGTCCTCAACATGGGTAAC | |||||||||||
Pp-UNICAMP09 | Lb-1 | KR611543 | (AC)7 | 56 | F: GCACAGGTAGAACCATTTCA | 228–260 | 8 | 0.81 | 0.85 | 6 | 0.78 |
R: TATGGAACTTGATGGGATTG | |||||||||||
Pp-UNICAMP10 | Lb-1 | KR611544 | (CA)7 | 60 | F: ATACCTTAGGATCCGCTTCA | 230–256 | 4 | 0.67 | 0.83 | 7 | 0.72 |
R: CACTCTACCGGTCCATGATA | |||||||||||
Pp-UNICAMP11 | Lb-1 | KR611545 | (CA)7 | 60 | F: GGAGAGATGAGACTCCCTTG | 232–266 | 6 | 0.72 | 0.86 | 5 | 0.69 |
R: ACTCTCTACCGGTCCATGAT | |||||||||||
Pp-UNICAMP12 | Lb-2 | KR611546 | (GT)7 | 65 | F: CGCGTGGACTACGATGG | 213–277 | 11 | 0.79 | 0.92 | 14 | 0.87 |
R: AAACGCCCACTCATAATTCG | |||||||||||
Pp-UNICAMP13 | Lb-2 | KR611547 | (CA)3CG(CA)3 | 55 | F: GGAGAGATGAGACTCCCTTGG | 116–142 | 10 | 0.71 | 0.74 | 8 | 0.63 |
R: TCAAGGTGGACCAAACACAC | |||||||||||
Pp-UNICAMP14 | Lb-2 | KR611548 | (ACAT)4 | 63 | F: GATGTTCCGCTCGTTTGATT | 223–243 | 5 | 0.59 | 0.75 | 8 | 0.67 |
R: TGTTGGTCTCAGGCAGCTTAT | |||||||||||
Pp-UNICAMP15 | Lb-2 | KR611549 | (AG)17 | 55 | F: ACAGCTTGGGCCTGACAC | 152–166 | 8 | 0.74 | 0.91 | 7 | 0.82 |
R: GGCTGAACTCTCTTCCATTGTT | |||||||||||
Pp-UNICAMP16 | Lb-2 | KR611550 | (AC)6 | 55 | F: GCACGTGTTCGTCATGAAAT | 258–300 | 4 | 0.33 | 0.33 | 9 | 0.69 |
R: GCTTGGTCCCATGGATTATG | |||||||||||
Pp-UNICAMP17 | Lb-2 | KR611551 | (AG)17 | 57 | F: TGACCGTGCCTAATCTCCTT | 147–191 | 15 | 0.87 | 0.99 | 13 | 0.86 |
R: AAGTTTGCTCTTTCGCGTGT | |||||||||||
Pp-UNICAMP18 | Lb-2 | KR611552 | (GT)8CA(GT)7 | 65 | F: CAGTCAACGACACGGGAAC | 119–153 | 11 | 0.84 | 0.98 | 16 | 0.90 |
R: CCCAACCTAAATCACCTCACC | |||||||||||
Pp-UNICAMP19 | Lb-2 | KR611553 | (ATT)2(CA)8 | 57 | F: CCCTCCCTCCATTTCACA | 203–241 | 13 | 0.87 | 0.97 | 15 | 0.88 |
R: AGCTCGCAGAAGGCAAGA | |||||||||||
Pp-UNICAMP20b | Lb-2 | KR611554 | (GT)7 | 65 | F: AAGAACTGCCAAGGAACT | 156–162 | 4 | 0.66 | 0.81 | 3 | 0.65 |
R: GGAATAAACCTCAATAGGG | |||||||||||
Pp-UNICAMP21 | Lb-2 | KR611555 | (CT)14 | 55 | F: GAGAGCCCAGACACAATGG | 145–205 | 14 | 0.88 | 0.99 | 17 | 0.90 |
R: ATCAACACGCTGCTTCAGTG | |||||||||||
Pp-UNICAMP22 | Lb-3 | KR611556 | (CA)3CG(CA)3 | 65 | F: CGCGGAGAGATGAGACT | 133–137 | 3 | 0.50 | 0.64 | 3 | 0.35 |
R: TCAAGGTGGACCAAACAC | |||||||||||
Pp-UNICAMP23 | Lb-3 | KR611557 | (TA)5A(GT)23 | 55 | F: AGCAGGAGATCATGGAG | 230–254 | 3 | 0.40 | 0.57 | 2 | 0.35 |
R: TCCTACGTGAATGGCTAC | |||||||||||
Pp-UNICAMP24 | Lb-3 | KR611558 | (CA)9 | 63 | F: TCTTGCATCATCCCGTATTG | 179–215 | 10 | 0.83 | 0.96 | 10 | 0.80 |
R: GCTGCCTGGTCCACTCTC | |||||||||||
Pp-UNICAMP25 | Lb-3 | KR611559 | (AC)8 | 63 | F: CGGACCGTCTTTATCTCCAA | 218–316 | 8 | 0.71 | 0.92 | 9 | 0.78 |
R: GCTCCGATCCTCGAAATTCT | |||||||||||