Fig. 2

Overexpression of Arg-I causes eNOS uncoupling, which is prevented by antioxidant NAC. Cells were transduced as described in Fig. 1. Forty-eight hours post-transduction, the cells were serum-starved for 16 h and then subjected to DHE and DAF-2DA staining for detection of O ^.−₂ (red) and NO (green), respectively. a Cells were treated with or without the eNOS inhibitor L-NAME (1 mmol/L, 1 h) during the last hour to demonstrate eNOS-uncoupling. b NAC (5 mmol/L) was added immediately after transduction and present in the culture medium until experiments were performed. Bar graphs in the corresponding right panels show quantification of the signals. *p < 0.05, **p < 0.01 and ***p < 0.005 vs. control (con); ^##<0.01 and vs. Arg-I. Scale bar  0.2 mm