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Table 1 Comparison of commercially available RNA isolation kits for use with PCLS

From: RNA isolation from precision-cut lung slices (PCLS) from different species

Material Kit RNA yield (ng) Absorbance 260/280 RIN
Avg. Min. Avg. Min. Avg. Min.
Freshly isolated cells from rat PCLS RNeasy Mini Kit, Qiagen 1146.5 855.0 2.04 1.99 7.8 7.4
Rat PCLS stored at −80 °C RNeasy Mini Kit, Qiagen <100 N/A N/A N/A N/A N/A
Rat PCLS stored at −80 °C QIAzol®, Qiagen 413.3 127.8 1.64 1.37 2.7a N/A
Rat PCLS stored at −80 °C MagJET, ThermoFisher 737.0 312.3 2.21 1.94 5.8 N/A
Rat PCLS stored at −80 °C MagMAX™, Ambion/ThermoFisher 1527.4 601.5 1.95 1.78 8.9 8.4
  1. Results represent the means of at least three samples
  2. N/A not available
  3. aN/A data were calculated as RIN zero (4 out of 6 samples)