Table 1 Comparison of commercially available RNA isolation kits for use with PCLS

Freshly isolated cells from rat PCLS

RNeasy Mini Kit, Qiagen

1146.5

855.0

2.04

1.99

7.8

7.4

Rat PCLS stored at −80 °C

RNeasy Mini Kit, Qiagen

<100

N/A

N/A

N/A

N/A

N/A

Rat PCLS stored at −80 °C

QIAzol^®, Qiagen

413.3

127.8

1.64

1.37

2.7^a

N/A

Rat PCLS stored at −80 °C

MagJET, ThermoFisher

737.0

312.3

2.21

1.94

5.8

N/A

Rat PCLS stored at −80 °C

MagMAX™, Ambion/ThermoFisher

1527.4

601.5

1.95

1.78

8.9

8.4