Table 1 13 Microsatellite loci, primer sequence, repeat motif, primer sets, PCR conditions and accession-numbers
Locus name | Primer sequence (5′–3′) | Repeat motif | Multiplex reaction | Labelling dye | Primer conc. | TA | Accession-Nr |
|---|---|---|---|---|---|---|---|
Mont_13187 | F: TGCCTGCAGTGCGTAGAG | CATA | R1 | VIC | 2 | 61/57 | KY094088 |
R: TATTGATTTCGGACAGGGCG | |||||||
Mont_13385 | F: GGTAAGCCAATAACGGTGGC | TCTT | R3 | PET | 2 | 58/51 | KY094089 |
R: ATCTGCAACGCCAGTAAACG | |||||||
Mont_17419 | F: ATAATGTGGGCAGAACAGGC | CAAA | R2 | 6-FAM | 0.5 | 58/51 | KY094090 |
R: GTCTTGGGTCGCACAGAATG | |||||||
Mont_2349 | F: TGACCCGCAGTGATCAAGTC | TTTC | R2 | VIC | 2 | 58/51 | KY094091 |
R: ATCCTATTTCGGTCCCTGGC | |||||||
Mont_2916 | F: CGTTGAATGAGTCACGGACG | GAAA | R3 | VIC | 3 | 58/51 | KY094092 |
R: ACACTTGTAGGCCGTTGTTC | |||||||
Mont_3056 | F: GAAAGAAGACCCCATTACGCC | AAAG | R1 | 6-FAM | 1 | 61/57 | KY094093 |
R: ATAGCGCACGTCTTTTGTCC | |||||||
Mont_3943 | F: CGATGATACAAGCAATGCGG | TCTT | R3 | NED | 2 | 58/51 | KY094094 |
R: CCATAGCTGCGTGCTTACAG | |||||||
Mont_4042 | F: AGCTAAAGTATCGTTGTATGGAGG | TCTT | R1 | PET | 2 | 61/57 | KY094095 |
R: CTCCGAGCATGAGCTTTCTG | |||||||
Mont_4196 | F: TCAGCCCTGCACAGATAGAC | AAGA | R2 | PET | 2 | 58/51 | KY094096 |
R: ACCTGGACAGCAGTTCTACG | |||||||
Mont_4477 | F: GTCTGACACAGGCAGCATAC | TTCT | R2 | 6-FAM | 4 | 58/51 | KY094097 |
R: CGTTGGTCTCCCGATTTCAC | |||||||
Mont_5483 | F: ATTCAATGTGCGCAAGTACG | TTTC | R1 | NED | 3 | 61/57 | KY094098 |
R: TGTCATTGATAGCCCCCTCC | |||||||
Mont_5717 | F: TATGGCCAACGAAACCAAGC | AATC | R3 | 6-FAM | 2 | 58/51 | KY094099 |
R: GTTCCGCATGGGACATGATAC | |||||||
Mont_5741 | F: TGGTATTGAGTGCATAGACGC | GTTT | R3 | 6-FAM | 2 | 58/51 | KY094100 |
R: CATTCTGGCGGGGATAAAGC |