Table 1 Nucleotide sequences for E. histolytica primers for stem LAMP test based on 18S rRNA and HLY6 genes
Target | Primer name | Sequence (5′–3′) | Bases | Final amplicon size |
|---|---|---|---|---|
18S rRNA gene | F3 | AAATACAAGGATAGCTTTGTG | 21 | Â |
B3 | AAGCTCCCTCTCCGATGTC | 19 | Â | |
FIP | CTCAATTCATTGAATGAATTGGCATGATAAAGATAATACTTGAGAC | 46 | 207 | |
BIP | CAATGAGAATTTCTGATCTATCCGTTATCCGTTATAATCTTGG | 43 | Â | |
LF | TTTGTACTAATACAAACTGGATC | 23 | Â | |
LB | CAGTTGGTAGTATCGAGGAC | 20 | Â | |
SF | CGACAATTGTAGAACACACAG | 21 | Â | |
SB | ATCCTAACTCACTTAGAATGTC | 22 | Â | |
HLY6 | F3 | ATACTTGAACGGATTG AAGCC | 21 | Â |
B3 | GTTTATTCATATGTTTGACAAGA | 22 | Â | |
FIP | CGCCCTATACTCAAATATGACACTTTGGTGGAAGATTCACG | 41 | 190 | |
BIP | AGGAAGGTCAAAGTATTAATAGTGTTGAGTGAATATACTCACC | 43 | Â | |
LF | GTAATTTGCACGTTAACACTG | 21 | Â | |
LB | TGGTAAAGATAATGATTAGGTG | 22 | Â | |
SF | CTGGTTCCACCTGAATATTC | 20 | Â | |
SB | TACTAGATAGTTCGATGAGTC | 21 | Â |