Fig. 3

The activity of cspA sequence::xylE reporter gene constructs in Staphylococcus aureus Newman. Units of XylE specific activity were measured over a 6 h time period with samples collected at 1, 3, and 6 h. The values for the cspA promoter::xylE plasmid pSK96 are represented by black-filled squares joined by red lines and the cspA promoter + 5′ UTR plasmid pSK192 values are open circles linked by blue lines. The 324 bp sequence::xylE reporter construct pSK324 is represented by black-filled diamonds connected by green lines. The XylE velocity for each time point was normalized by subtraction of the velocity of the promoterless xylE reporter plasmid pLL38 before the units specific activity (nmol/min/mg protein) were calculated. Double-ended error bars represent the standard error of the mean for each time point