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Fig. 8 | BMC Research Notes

Fig. 8

From: Immuno-diagnosis of Mycobacterium tuberculosis in sputum, and reduction of timelines for its positive cultures to within 3 h by pathogen-specific thymidylate kinase expression assays

Fig. 8

TMKmt Ag levels in serial dilutions of 1 + acid fast bacilli ladden patient sputum sample detected. This figure shows TMKmt Ag levels in serial dilutions of 1 + acid fast bacilli ladden patient sputum sample detected by PAb-0655. Direct Enzyme Immuno-assay (EIA) detection of TMKmt Ag using two custom polyclonal antibodies (PAb-0655 and PAb-0656) in the 1 × 10−1, 1 × 10−2, 1 × 10−3, 1 × 10−4, 1 × 10−5, 1 × 10−6 dilutions of sputum containing (considering the initial 1+ AFB sputum with 105 CFUs/ml) approximately 104, 103, 102, 10, 1, and 0.1 CFUs/ml of acid fast bacilli. Specifically, TMKmt antigen was detected up to 1 × 10−4 to 1 × 10−5 (containing 10 and 1 CFUs/ml) dilutions of a bead-treated, microscopically designated 1+ (est. Acid Fast Bacillary load of 1 × 105) patient sample by PAb-0655 and PAb-0656, respectively

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