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Table 1 Real-time PCR amplification scores versus visual observations

From: The detection of great crested newts year round via environmental DNA analysis

  eDNA score 1 eDNA score 2 eDNA score 3 Visual observation
Pond 1 March 7/12 3/12 6/12 Present
Pond 2 March 11/12 11/12 12/12 Present
Pond 1 April 12/12 12/12 11/12 Present
Pond 2 April 12/12 12/12 11/12 Present
Pond 1 May 6/12 9/12 3/12 Present
Pond 2 May 11/12 9/12 9/12 Present
Pond 1 June 5/12 4/12 4/12 Present
Pond 2 June 12/12 11/12 12/12 Present
Pond 1 July 6/12 11/12 9/12 Present
Pond 2 July 12/12 12/12 12/12 Present
Pond 1 August 7/12 7/12 8/12 Present
Pond 2 August 12/12 12/12 11/12 Present
Pond 1 September 1/12 0/12 0/12 Absent
Pond 2 September 3/12 2/12 0/12 Absent
Pond 1 October 0/12 0/12 0/12 Absent
Pond 2 October 0/12 2/12 0/12 Absent
Pond 1 November 0/12 1/12 0/12 Absent
Pond 2 November 1/12 4/12 1/12 Absent
Pond 1 December 1/12 0/12 1/12 Absent
Pond 2 December 0/12 0/12 0/12 Absent
Pond 1 January 6/12 0/12 3/12 Absent
Pond 2 January 2/12 3/12 4/12 Absent
Pond 1 February 6/12 4/12 5/12 Present
Pond 2 February 2/12 0/12 0/12 Present
  1. Real-time PCR amplification scores (numbers of individual PCRs positive out of a maximum of 12 replicates) for eDNA extracted from water samples taken monthly from two ponds over a year. eDNA was measured using PCR × 12 replicates for each of three separate PCR amplifications of a single eDNA extraction. Visual observations were made prior to collection of the water samples every month and great crested newts recorded as present or absent