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Table 2 Summary of immunohistochemistry and immunoblot results

From: Authentication of collagen VI antibodies

Antibody Cat number Signal by IHC α1 lysate α2 lysate α3 lysate α1 α2 α3 lysate Figures
COL6A1 H200 sc-20649 ++ ++ (R) + 1
COL6A1 ab199720 +++ +++ (R)/+++ (NR) + 1
COL6A1 ab182744 ++ ++ (R) + 1
COL6A1 17023-1-AP +++ +++ (R)/+++ (NR) + 1
COL6A1 B4 sc-377143 +++ (R) + 1
COL6A2 14853-1-AP ++ ++ (R)/+ (NR) nd 2
COL6A2 EPR7889 ab180855 +++ +++ (R) 2
COL6A2 ab172606 ++ + (R) nd 2
COL6A2 D20 sc-83607 +++ +++ (R) ++ 2
COL6A2 H300 sc-292186 ++ + (R) + 2
COL6A2 K15 sc-377143 +++ + (R) + 2
COL6A2 B7 sc-374566 +++ (R)/+ (NR) ++ 2
COL6A3 G18 sc-131139 ++ + (NR) + 3
COL6A3 3C4 MAB1944 +++ + (R)/++ (NR) 3
COL6A3 3C4 sc-47712   + nd 3
COL6A3 H300 sc-367543 +++ 3
COL6A3 H3-2 sc-81766 3
COL6A3 64C11 ab49273 3
COL6A3 N12 sc-131140 nd 3
Collagen VI VI-26 MAB3303 +++ + 4
Collagen VI 70R-CR009X +++ ++ (NR) +++ 4
Collagen VI 172C2 sc-47764 ++ (NR) 4
Collagen VI 5C6   + 4
Collagen VI AB7821 Non-sp. Non-sp. ++ 4
  1. Each antibody is scored for signal intensity on immunohistochemical signal and, for immunoblots, band intensity ranging from ‘−’ representing no detectable signal, to ‘+++’ representing strong signal. The description of immunoblots signal strength scores reduced (R) and non-reduced (NR) samples separately. Some blots on triple transfected lysates were not conducted and are designated as not determined (nd)