Skip to main content

Table 1 Performance of Vikia® Rota-Adeno and Premier™ Rotaclone ® compared to RT-PCR on frozen samples

From: Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger

  RT-PCR Test performance
Positive Negative Total % sensitivity (95% CI) % specificity (95% CI)
Vikia Rota-Adeno 80.7 (72.4–87.3) 95.5 (90.4–98.3)
 Positive 96 6 102
 Negative 23 126 149
Premier Rotaclone 80.7 (72.4–87.3) 100 (97.2–100)
 Visual reading
  Positive 96 0 96
  Negative 23 131 154
  Indeterminate 0 1 1
 Optical density
  Positive 96 0 96
  Negative 23 132 155
Total 119 132 251
  1. In order to assess possible sample selection bias and freezing/thawing impact on sample rotavirus status, a comparison of the initial VIKIA® Rota-Adeno test in the field for the purposes of surveillance with RT-PCR results on all specimens tested and those selected for the evaluation are shown in Table 2. There was no significant difference between the sensitivity and specificity of the RDT when calculated using all specimens or only those selected for the evaluation, suggesting absence of sample selection bias