Skip to main content

Advertisement

Table 4 Determination of markers of hAECs by flow cytometry

From: Method and key points for isolation of human amniotic epithelial cells with high yield, viability and purity

Marker Donor1 Donor2 Donor3 Donor4 Donor5 Donor6
Pan cytokeratin ++++ ++++ ++++ ++++ ++++ ++++
CD73 +++ +++ +++ +++ +++ +++
SSEA-4 +++ +++ +++ +++ +++ +++
CD133 - - - - - -
CD90 - - - - - -
CD105 - - - - - -
CD34 - - - - - -
CD45 - - - - - -
CD9 ++ - + + - +
CD38 - - - - - -
HLA-DR - - - - - -
Integrin-β2 (CD29) + + + + ++ +
Hyaluronic acid receptor (CD44) - + + + ++ +
CD56 -/+ -/+ -/+ -/+ -/+ -/+
CD14 + ++ - + ++ -
CD3 - - - - - -
CD4 - - - - - -
CD8 - - - - - -
  1. –, Not determined; , very low expression (< 10%); +, low expression (10–30%); ++, intermediate expression (30–60%); +++, high expression (60–90%); ++++, very high expression (> 90%)