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Table 2 List of reagents used in this work

From: Absence of nuclease activity in commonly used oxygen-scavenging systems

Reagent Senavirathne et al. Gahlon et al.
Buffer 50 mM Tris–HCl 50 mM Tris–HCl
pH 7.5 7.5
NaCl (mM) 100 100
MgCl2 (mM) 5 5
DTT (mM) 0.1 0.1
Incubation time (min) 40 40
Temperature (°C) 22 22
PCA (mM) 5 5
PCD 0.21 U/mL 60 nMa
Glucose, (%) 0.8 1
GOD (U/mL) 165 35
Catalase (U/mL) 2170 62,500
Linear DNA 10 ng/µLb 100 nM
Plasmid (ng/µL) 10 10
  1. aWe used conditions comparable to their highest concentration
  2. bConcentration varies from ~ 350 to 600 nM depending on construct size