Table 2 List of reagents used in this work
From: Absence of nuclease activity in commonly used oxygen-scavenging systems
Reagent | Senavirathne et al. | Gahlon et al. |
|---|---|---|
Buffer | 50 mM Tris–HCl | 50 mM Tris–HCl |
pH | 7.5 | 7.5 |
NaCl (mM) | 100 | 100 |
MgCl2 (mM) | 5 | 5 |
DTT (mM) | 0.1 | 0.1 |
Incubation time (min) | 40 | 40 |
Temperature (°C) | 22 | 22 |
PCA (mM) | 5 | 5 |
PCD | 0.21Â U/mL | 60Â nMa |
Glucose, (%) | 0.8 | 1 |
GOD (U/mL) | 165 | 35 |
Catalase (U/mL) | 2170 | 62,500 |
Linear DNA | 10 ng/µLb | 100 nM |
Plasmid (ng/µL) | 10 | 10 |