Fig. 1

Growth kinetics of C. glutamicum and E. coli from BioLector cultivations and depiction of data processing for automated growth rate calculation. a Online monitored backscatter and dissolved oxygen (DO) signal for C. glutamicum in CgXII medium with 10 g/L glucose, a filling volume of 1000 µL and a shaking frequency of 1000 rpm. The panel on the right shows the processed biomass data, i.e. blanked backscatter and data points determined automatically for calculation of growth rate. Propagated measurement error for blanked BS signal was calculated to 0.39 a.u. b Online monitored signals like in part a, but for E. coli in M9 medium with 20 g/L glucose, a filling volume of 1000 µL and a shaking frequency of 1400 rpm. Propagated measurement error for blanked BS signal was calculated to 0.34 a.u. Right panel analogous to the one in part A. Insets in part B magnify the first 18 h of cultivation. Measurement cycle time for recording backscatter and DO signals was set to 9 min for both C. glutamicum and E. coli cultivations