Table 1 Summary information for the 15 microsatellite markers used in this study of Vulpes lagopus
From: Fine-scale population genetic structure of arctic foxes (Vulpes lagopus) in the High Arctic
Locus | Primer sequences (5′ to 3′) | Repeat motif | Size range (bp) | Number of alleles | Ho (He) | PCR multiplex | Ta (°C) | F IS | F null |
|---|---|---|---|---|---|---|---|---|---|
CPH5 [12] | F:FAM-TCC ATA ACA AGA CCC CAA AC | (TG)17 | 84–112 | 14 | 0.788 (0.845) | 1 | 57.4 | 0.070 | 0.038 |
R:GGA GGT AGG GGT CAA AAG TT | |||||||||
CPH9 [12] | F:PET-CAG AGA CTG CCA CTT TAA ACA CAC | (GT)18 | 152–164 | 6 | 0.671 (0.667) | 1 | 57.4 | − 0.004 | 0.001 |
R:AAA GTT CTC AAA TAC CAT TGT GTT ACA | |||||||||
CPH15 [12] | F:FAM-GCC TAT ATA AAA TGC ATC TGA GC | (AC)18 | 111–139 | 14 | 0.876 (0.890) | 1 | 57.4 | 0.017* | 0.015 |
R:CCG TGA CTC CTG TCT TCT GAC | |||||||||
CXX147 [13] | F:NED-CCA TGG GAA ACC ACT TGC | (AC)20 | 168–188 | 11 | 0.852 (0.842) | 1 | 57.4 | − 0.010* | 0.009 |
R:ACT TCA TCA TGT CTG GAA GCG | |||||||||
CXX173 [13] | F:NED-ATC CAG GTC TGG AAT ACC CC | (TG)17 | 124–132 | 5 | 0.667 (0.739) | 1 | 57.4 | 0.100 | 0.039 |
R:TCC TTT GAA TTA GCA CTT GGC | |||||||||
CXX250 [13] | F:PET-TTA GTT AAC CCA GCT CCC CCA | (AC)18A2(TC)4 | 120–140 | 11 | 0.862 (0.862) | 1 | 57.4 | 0.007 | 0.009 |
R:TCA CCC TGT TAG CTG CTC AA | |||||||||
CXX733 [15] | F:VIC-CCCTCTACTTATGTCTCGGCC | Dinucleotide | 245–271 | 12 | 0.576 (0.706) | 1 | 57.4 | 0.187* | 0.090 |
R:GAGAGGAGAAACAACCAACACC | |||||||||
CXX758 [15] | F:NED-AAG CAT CCA GAA TCC CTG G | Dinucleotide | 213–253 | 17 | 0.857 (0.872) | 1 | 57.4 | 0.019 | 0.020 |
R:GTT GAT TGG GAG ATA ATC CAC A | |||||||||
CXX771 [15] | F:VIC-GAG GAA GCC TAT GGT AGC CA | Dinucleotide | 85–123 | 17 | 0.824 (0.851) | 1 | 57.4 | 0.034* | 0.018 |
R:CAA GAC CTG AAT TCC TTG TTC C | |||||||||
CPH3 [12] | F:NED-CAG GTT CAA ATG ATG TTT TCA G | (GA)2TA(GA)17 | 156–188 | 16 | 0.886 (0.888) | 2 | 49.9 | 0.005 | 0.010 |
R:TTG ACT GAA GGA GAT GTG GTA A | |||||||||
CXX20 [13] | F:PET-AGC AAC CCC TCC CAT TTA CT | (CA)21 | 121–139 | 10 | 0.823 (0.848) | 2 | 49.9 | 0.031 | 0.003 |
R:TTG ATC TGA ATA GTC CTC TGC G | |||||||||
CXX140 [13] | F:FAM-CAG AGG TGG CAT AGG GTG AT | (GT)15 | 137–161 | 11 | 0.790 (0.815) | 2 | 49.9 | 0.033 | 0.009 |
R:TCG AAG CCC AGA GAA TGA CT | |||||||||
CXX745 [15] | F:FAM-TTT ATG GTC TCC ATG AGG GC | Dinucleotide | 267–283 | 9 | 0.866 (0.850) | 2 | 49.9 | − 0.016 | 0.000 |
R:TCC CTG CAT TTC CTT ATT TCA | |||||||||
CXX671 [14] | F:VIC-AAA ATG AAA AAG GAA GAG AGC | (CA)10 | 199–215 | 9 | 0.880 (0.840) | 2 | 49.9 | − 0.044 | 0.000 |
R:AGG AGA CAG GAT TTT CCT CTC A | |||||||||
CXX377 [14] | F:VIC-ACG TGT TGA TGT ACA TTC CTG | (AC)12 | 173–193 | 11 | 0.843 (0.863) | Singleplex | 58 | 0.026 | 0.016 |
R:CCA CCC AGT CAC ACA ATC AG |