Fig. 1

Exposure to green leaf volatiles activated the 46 and 44 kDa MAPKs in Lt. a Immunoblots showing the activation of the Lt MAPKs. 3X wound—Lt plants were wounded 3 times perpendicular across all tillers 1–2 in. apart, spaced vertically from the base of the plants, using a hemostat. Three-four week-old Lt plants at the two tiller stage were exposed GLV by placing them in a closed clear (non-air tight) cylinder with freshly cut and damaged Lt leaf tissue for the entire 60 min of the time course or for only 1 min. Control treatment plants were placed into the cylinders without any grass clippings present. b Immunoblots of LT plant extracts of time points taken from: 10W, 10 min after wounding; 3GLV, 3 min after GLV exposure; 20W, 20 min after wounding; 25GLV, 25 min after GLV exposure; were separated on adjacent lanes on a SDS-PAGE gel. One plant per time point was collected at the times indicated. To determine MAPK activity, immunoblots of protein extracts were performed using anti-phospho-MAPK (Erk1/2) antibody. The experiments were independently repeated three times. Representative blots are presented. Note while previously the MAPK bands were designated as 46 and 44 kDa MAPKs based on a comparison of their migration distance with protein molecular weight markers on SDS-PAGE gels, but their actual molecular weight range may be 46–47 and 43–44 kDa