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Table 1 GMO detection of food products according to group type

From: Comparison of three DNA extraction methods for the detection and quantification of GMO in Ecuadorian manufactured food

Food groups

Code

Number of samples

Endogenous genes

Endogenous genes detectiona

Transgenic event detection

Conventional method

Commercial kits

Qualitative PCR P35S and t-NOSb

Percentage of GTS 40-3-2 event in whole food productc

Percentage of MON810 in whole food productc

CTAB

Wizard® Magnetic DNA Purification System for Food (PROMEGA)

Dneasy mericon food (QIAGEN)

P35S positive

Tnos positive

P35S and Tnos positive

Code group

%

Code group

%

Flour

F

10

Lectin

5

5

7

9

5

5

F1

0.1

F2

0.1

F3

1.8

Alcohol dehydrogenase

4

5

4

F6

4.7

F6

3.7

F8

2.3

Cereal

C

4

Lectin

2

1

3

4

0

0

C1

1.1

  

Alcohol dehydrogenase

1

0

1

  

Grain

G

3

Lectin

1

1

3

3

0

0

G3

0.1

  

Alcohol dehydrogenase

2

2

1

  

Snacks

Sk

4

Lectin

0

0

3

4

1

1

n/a

n/a

  

Alcohol dehydrogenase

2

4

1

n/a

n/a

  

Sausage

S

14

Lectin

11

9

14

6

2

2

S1

2.3

  

S2

2.1

  

Alcohol dehydrogenase

0

3

1

S3

4.2

  

S5

2.3

  

S13

1.9

  

Total

 

35

     

26

8

8

    
  1. aPCR amplification of lectin and alcohol dehydrogenase genes
  2. bP35S refers to 35S promoter from the Cauliflower mosaic virus and Tnos refers to nopaline synthase terminator from Agrobacterium tumefaciens
  3. cGTS 40-3-2 and MON810 quantification with qPCR