Fig. 2
Peptide-specific CTL responses (cytotoxicity) in three A18+ cattle. BE017 (a), BE033 (b) and BE043 (c) and one control calf BE029 (d). CTL from the cattle were tested for killing on autologous cell lines infected with Muguga or Marikebuni (e.g. BE017-Mug or BE017-Mar) or autologous PBMC pulsed with the Tp1214–224 peptide from T. parva Muguga (Tp1 Mug) or T. parva Marikebuni (Tp1 Mar), as indicated. The experiment was performed twice with similar results. T-test was used for testing statistical significance. The highest E:T ratio was tested against the PBMC control. *P < 0.05, **P < 0.01, ***P < 0.001. Significance between killing of Muguga and Marikebuni was also tested and found to be significantly less (for Marikebuni) with at least P < 0.05 for the animals BE033, BE043 and BE029 but not for BE017